Wednesday Experiments 1 DNAfingerprinting 2 Extraction of DNA

Wednesday: Experiments: 1: DNA-fingerprinting 2: Extraction of DNA 3: Microbiology: Assaying bacteria in our environment Thursday: Results from: 1: DNA-fingerprinting 2: Microbiology: Assaying bacteria in our environment Experiments: 4: Microbiology: Growth Factors and yeast 5: Blood: Testing for blood types 02 -01 -2022 1

• Crime Scene Report • Location: Aalborghus Gymnasium, teachers’ preparation room • Date: January the 31 th, 07: 30 AM 02 -01 -2022 2

Primary suspects Suspect 1: Ole Suspect 3: Rolf Suspect 2: Thorbjørn Suspect 4: Torben 3

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Chromosomes and DNA 02 -01 -2022 5

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Variations i DNA SNP’s: Small Nuclear Polymorphisms STR’s: Small Tandem Repeats STR’s gives the opportunity to separate them by size 02 -01 -2022 7

A person inherit a copy (allele) of a STR region from each of the parents HOMOZYGOTE: both alleles are of the same length (same number of repeats) HETEROZYGOTE: the alleles are of different length (unevenly number og repeats) 02 -01 -2022 8

CODIS (FBI 1997) Combined DNA Index System 02 -01 -2022 9

Procedure 1. 2. 3. 4. Get your samples (handed out to you) Extract DNA (already extracted) PCR: Multiply STR’s Gel Electrophoresis: Separate STR’s 5. Stain DNA 6. View results –Compare suspects with Crime Scene DNA 02 -01 -2022 10

PCR You add: • Sample DNA • TAQpolymerase • Primers 1 &2 • nucleotides 02 -01 -2022 Indsæt note og kildehenvisning via 11

Gel electroforesis 02 -01 -2022 Indsæt note og kildehenvisning via 12