Ways to Regulate Transcription Alternate sigma factor usage
- Slides: 45
Ways to Regulate Transcription Alternate sigma factor usage: controls selective transcription of entire sets of genes vegetative (principal s) s 70 צמיחה heat shock nitrogen starvation (16 -19 bp) TTGACA s 32 s 60 +1 TATAAT (5 -9 bp) A +1 CNCTTGA (13 -15 bp) CCCATNT (5 -9 bp) A CTGGNA (6 bp) TTGCA +1 (5 -9 bp) A !19. 6. 2008 – התרגיל ביום חמישי זה
Pre-m. RNA * micro. RNAs (mi. RNA) RNA molecules of about 21– 23 nucleotides in length, which regulate gene expression. are processed from primary transcripts known as pri-mi. RNA.
5’UTR 3’UTR
RNA polymerase
שלבי הטרנסקריפציה Initiation • Elongation • Termination •
Stages in Initiation of Transcription • Bacterial transcription • Closed complex: holoenzyme+promoter • Open complex (DNA melting, not need ATP) • Abortive transcripton • Productive initiation – Transcribe past +9 – Sigma dissociates • Elongation • Eukaryotic transcription • Preinitiation complex (PIC) assembly • PIC activation (DNA melting, needs ATP) • Abortive transcription • Productive initiation – CTD phosphorylated – Promoter clearance • Elongation
initiation
Elongation NTPs
TERMINATION • RNA polymerase meets the terminator • Terminator sequence: AAUAAA • RNA polymerase releases from DNA • Prokaryotes-releases at termination signal • Eukaryotes-releases 10 -35 base pairs after termination signal
transcription
Transcription regulation
Gene expression
Basics of cell biology: development Fertilized egg
Basics of cell biology: development Cells differentiation is due to different gene Switches going on and off These cells are different because they express a subset of their 24, 000 genes
Promoter Gene To specify a new cell: place it in the right environment or
EGF Hedgehog Wnt Promoter Gene Retinoic acid FGF HGF To specify a new cell: place it in the right environment or Substitute drugs/proteins for environment Mophogens
Chromosome localization in interphase In interphase, chromosomes appear to be localized to a sub-region of the nucleus.
- Sigma sigma phi vcom
- Vcom mission statement
- Gods ways are not our ways
- Centrifuge sigma factor
- Defeat two factor authentication
- Co regulate
- Apotema piramidei formula
- Thermoneutral
- Regulate public conduct and set out duties owed to society
- Corpuri geometrice in spatiu
- Proiectia ortogonala
- Apotema
- Inaltimea piramidei
- Prisma desfasurata
- Mla format regulates
- Autonomic motor neurons regulate visceral activities by
- Formule poligoane regulate
- An uncontrolled railroad crossing
- Does the urinary system regulate blood pressure
- H in tetraedrul regulat
- Factoring greatest common factor
- Factor out the greatest common factor
- Greatest common factor of 20 and 36
- Form factor formula
- What is factor isolating questions
- Gcf of expressions
- Translation transcription
- Transcription
- Activation domain of transcription factors
- Akaptonuria
- Read the transcription and write the words
- Idea transcription
- Dna to rna transcription
- Word transcription
- Rna transcription
- Transcription ocr
- Dna transcription
- Transcription
- Translation transcription
- Prokaryotic
- Acids and bases venn diagram
- The sounds of language
- Perbedaan replikasi virus dna dan rna
- Transcription inhibitors
- Translate
- Replication