Watson et al BIOLOGIA MOLECOLARE DEL GENE Zanichelli

hn. RNP § Circa 30 proteine abbondanti identificate su gel bidimensionale § Contengono motivi di legame all’RNA (RRM) e altri domini “ausiliari” § I complessi hn. RNP contengono circa 700 nt di RNA § Molte hn. RNP fanno da “navetta” tra nucleo a citoplasma

Tipi di splicing meccanismo simile • Splicing nucleare • Splicing di introni di tipo II spliceosoma autosplicing transesterificazioni • Splicing di introni di tipo I • Splicing di t. RNA di lievito nucleasi e ligasi

sn. RNP nello spliceosoma • U 1 • U 2 • U 4/U 6 • U 5

Figure 2 Potential base pairing interactions between the branch site of the human P 120 AT–AC intron and U 12 or U 2 sn. RNAs. The adenosine residue that forms the lariat structure is marked by an asterisk and shown bulged from the putative intron–sn. RNA hybrid. Possible base pairings are indicated by vertical lines. The sites in the P 120 intron where mutations inhibit splicing in vivo are marked in bold, as are the corresponding two sites in U 12 sn. RNA where compensatory mutations that restore the potential for base pairing suppress the splicing defect