WASTE DISPOSAL FLOWCHART Does the waste contain any

WASTE DISPOSAL FLOWCHART Does the waste contain any radioactive materials? YES Contact Radiation Safety Officer for assistance x 6771 NO Does the waste contain any hazardous chemicals (formaldehyde, ethidium bromide, flammable liquids, etc. ), or is the p. H of the material <5 or >10? YES NO Does the waste contain material of human or animal origin (blood, blood products, tissues, cell lines)? Is waste contaminated with any material of human or animal origin? YES Does the waste contain cultures of bacteria, recombinant DNA organisms, viruses, or material that is contaminated with such agents (pipet tips, gloves, microfuge tubes, etc. )? x 5617 YES Collect sharps in punctureresistant container marked “Sharps”. If bacterial or viral agents are present, place sharps container in autoclave bag and follow Autoclaving Flowchart NO NO Contact Incinerator Supervisor for disposal x 7020 NO Does the waste contain sharps (syringes, razor blades, Pasteur pipets, microscope slides)? Contact UAF Hazmat for disposal or assistance YES If no bacterial or viral agents are present, remove any “Biohazard” labels and place sharps container in box marked “Broken Glass”. Seal and dispose in regular trash. See Autoclaving Flowchart Material can be disposed with regular trash or down the drain

AUTOCLAVING FLOWCHART STOP! Do the items that you want to autoclave contain any of the following: flammable liquids (ethanol, methanol, acetone, etc. ) toxic or reactive chemicals bleach radioactive isotopes animal carcasses or large blocks of tissue NO Are you autoclaving liquids or a combination of liquids and solid items? Place items in a tray and ensure that caps are not tightly sealed (containers may explode). Separate items to allow steam circulation. YES NO Are you autoclaving only solid items, such as new pipet tips, empty glassware, Pasteur pipets, forceps, scissors, etc. ? NO Are you autoclaving solid waste in an autoclave bag, such as contaminated agar plates, gloves, tips, swabs? YES x 6771 or x 5617 Is the maximum volume in any given tube or bottle <500 m. L? NO Is the maximum volume in any given tube or bottle >500 m. L? YES Do not autoclave these materials. Contact EHS&RM for assistance. YES Autoclave for 20 min on liquid cycle. Autoclave for 35 min on liquid cycle. NOTE: very large volumes may require longer times. Place items on tray and ensure that any lids are not tightly sealed. Separate objects so that steam can circulate completely around them. Autoclave for 20 minutes using a gravity cycle. YES Place bag in bin or tray. Add 100 -200 m. L water to bag just prior to placing in autoclave (to generate steam within bag during cycle). Close bag loosely. Autoclave for 35 -60 minutes using a gravity cycle. After cycle is finished, place bag in gray Autoclaved Waste Disposal bin next to autoclave.