Wakulla Springs Alliance Dye Studies Sean Mc Glynn

Wakulla Springs Alliance Dye Studies Sean Mc. Glynn, Mc. Glynn Labs, LLC, Principal Investigator Robert Deyle, Project Manager Funded by grant PFS 1617 -08 from the Fish and Wildlife Foundation of Florida

Methods • Dye used: 100 lbs of ~20% liquid rhodamine WT dye • Sampling and analysis • In situ sample readings every 10 minutes with Hydrolab MS 5 sondes equipped with rhodamine WT submersible fluorimeter sensors • Factory-calibrated Turner Designs Cyclops-7 submersible fluorimeters with detection range of 0. 01 to 1, 000 μg/L (ppb) – swapped out weekly • Sensors placed about a month before expected arrival of dye • Discrete grab samples were measured for background fluorescence weekly for another two months prior to deploying the sensors in the field • All grab sample background levels were below detection limit

Wakulla Spring Sonde Fluorometer Results Dye Injection Site Lake Jackson Porter Hole sink Upper Lake Lafayette 1 Fallschase sink Upper Lake Lafayette 2 Lake Iamonia Fallschase sink Iamonia sink Date of Dye Injection Date In Initial Detection Date Maximum Concentration (μg/L) 9/19/17 9/21/17 10/24/17 6. 31 1/19/17 2/23/17 0. 24 4/9/18 4/12/18 4/16/18 5. 00 7/16/18 7/5/18 7/31/18 ~14. 5 Transit Time Second pulse Maximum Concentration (μg/L) 35 days n/a n/a 7 days 5/9/18 123. 88 15 days 8/5/18 0. 7 Transit Time n/a 30 days 20 days

Wakulla Spring Sonde Fluorometer Results Dye Injection Site Lake Jackson Porter Hole sink Upper Lake Lafayette 1 Fallschase sink Upper Lake Lafayette 2 Lake Iamonia Fallschase sink Iamonia sink Date of Dye Injection Date In Initial Detection Date Maximum Concentration (μg/L) 9/19/17 9/21/17 10/24/17 6. 31 1/19/17 2/23/17 0. 24 4/9/18 4/12/18 4/16/18 5. 00 7/16/18 7/5/18 7/31/18 ~14. 5 Transit Time Second pulse Maximum Concentration (μg/L) 35 days n/a n/a 7 days 5/9/18 123. 88 15 days 8/5/18 0. 7 Transit Time n/a 30 days 20 days

Lake Jackson Porter Hole Sink Injection Site

Wakulla Spring Dye Detection 9/29/17 11/07/17: Lake Jackson Dye Study Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 9/19/17 9/21/17 10/24/17 6. 31 35 days

Wakulla Spring Dye Detection 10/24/17: Lake Jackson Dye Study Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 9/19/17 9/21/17 10/24/17 6. 31 35 days

Sally Ward Spring Dye Detection 10/12/17 10/22/17: Lake Jackson Dye Study Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 9/19/17 10/04/17 10/20/17 3. 25 31 days

Wakulla Spring Sonde Fluorometer Results Dye Injection Site Lake Jackson Porter Hole sink Upper Lake Lafayette 1 Fallschase sink Upper Lake Lafayette 2 Lake Iamonia Fallschase sink Iamonia sink Date of Dye Injection Date In Initial Detection Date Maximum Concentration (μg/L) 9/19/17 9/21/17 10/24/17 6. 31 1/19/17 2/23/17 0. 24 4/9/18 4/12/18 4/16/18 5. 00 7/16/18 7/5/18 7/31/18 ~14. 5 Transit Time Second pulse Maximum Concentration (μg/L) 35 days n/a n/a 7 days 5/9/18 123. 88 15 days 8/5/18 0. 7 Transit Time n/a 30 days 20 days

Upper Lake Lafayette Fallschase Sink Injection Site

Wakulla Spring Dye Detection 1/26/17 2/23/17: ULL Dye Study #1 Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 1/19/17 2/23/17 0. 24 35 days

Wakulla Spring Sonde Fluorometer Results Dye Injection Site Lake Jackson Porter Hole sink Upper Lake Lafayette 1 Fallschase sink Upper Lake Lafayette 2 Lake Iamonia Fallschase sink Iamonia sink Date of Dye Injection Date In Initial Detection Date Maximum Concentration (μg/L) 9/19/17 9/21/17 10/24/17 6. 31 1/19/17 2/23/17 0. 24 4/9/18 4/12/18 4/16/18 5. 00 7/16/18 7/5/18 7/31/18 ~14. 5 Transit Time Second pulse Maximum Concentration (μg/L) 35 days n/a n/a 7 days 5/9/18 123. 88 15 days 8/5/18 0. 7 Transit Time n/a 30 days 20 days

Wakulla Spring Dye Detection 4/12/18 6/4/18: ULL Dye Study #2 Initial Pulse Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 4/9/18 4/12/18 4/16/18 5. 00 7 days

Wakulla Spring Dye Detection 4/12/18 6/4/18: ULL Dye Study #2 Second Pulse Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 4/9/18 4/12/18 5/9/18 123. 88 30 days

Buck Lake Fallschase Sink 0. 25 mile

Dye backup into Upper Lake Lafayette after draining of Buck Lake, April 17, 2018

Sally Ward Dye Detection 4/20/18 - 4/29/18: ULL Dye Study #2 Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 4/9/18 4/20/18 21. 64 ≤ 11 days

Wakulla Spring Sonde Fluorometer Results Dye Injection Site Lake Jackson Porter Hole sink Upper Lake Lafayette 1 Fallschase sink Upper Lake Lafayette 2 Lake Iamonia Fallschase sink Iamonia sink Date of Dye Injection Date In Initial Detection Date Maximum Concentration (μg/L) 9/19/17 9/21/17 10/24/17 6. 31 1/19/17 2/23/17 0. 24 4/9/18 4/12/18 4/16/18 5. 00 7/16/18 7/5/18 7/31/18 ~14. 5 Transit Time Second pulse Maximum Concentration (μg/L) 35 days n/a n/a 7 days 5/9/18 123. 88 15 days 8/5/18 0. 7 Transit Time n/a 30 days 20 days

Lake Iamonia

Iamonia Sink

Wakulla Spring Dye Detection 7/26/18 8/9/18: Lake Iamonia Dye Study Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 7/16/18 7/5/18 7/27/18 ~14. 50 11 days

Sally Ward Dye Detection 7/18/18 - 8/7/18: Lake Iamonia Dye Study Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 7/16/18 ? ? ? 7/21/18 0. 02 5 days

Sally Ward Dye Detection 8/9/18 - 8/24/18: Lake Iamonia Dye Study: Second Pulse Injection Date In Initial Detection Date Maximum Concentration (μg/L) Transit Time 7/16/18 7/18/18 8/22/18 ~2. 75 35 days