Vincent A Chiappinelli Ph D The Ralph E

FUNCTIONAL PROPERTIES OF NEURONAL NICOTINIC RECEPTORS NIH Competitive Renewal – 21 st Year Starts

Patch-Clamp Recording from Visualized Nerve Cells in Living Brain Slices Allows Real-Time Recording of

Electrical Stimulation of Cholinergic Fiber Tract: Postsynaptic nicotinic response in SPL neuron In Presence

Ventral Lateral Geniculate Neuron (LGNv) ACh increases evoked glutamatergic currents by 3 -fold through

Schematic diagram of selected circuitry within the ventral Lateral Geniculate Nucleus (LGNv) a 4*

125 I-a-Conotoxin MII autoradiography in rat brain: LGN and optic tract heavily labeled revealing

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Vincent A. Chiappinelli, Ph. D. The Ralph E. Loewy Professor Chair, Dept. of Pharmacology & Physiology Tel: 202 -994 -3541 phmvac@gwumc. edu

FUNCTIONAL PROPERTIES OF NEURONAL NICOTINIC RECEPTORS NIH Competitive Renewal – 21 st Year Starts Dec 2006 MAIN GOAL: Combine electrophysiological and morphological methods to determine roles of nicotinic receptors (n. ACh. Rs) in synaptic transmission in the CNS during development Principal Investigator: Vince Chiappinelli Co-Principal Investigator: Jian-Zhong Guo Co-Principal Investigator: Eva Sorenson Consultant: David Perry Consultant: Michael Mc. Intosh (Univ. Utah)

Patch-Clamp Recording from Visualized Nerve Cells in Living Brain Slices Allows Real-Time Recording of Synaptic Responses

Electrical Stimulation of Cholinergic Fiber Tract: Postsynaptic nicotinic response in SPL neuron In Presence of GABA and Glutamate ELECTRICAL STIMULATION OF CHOLINERGIC INPUTS HERE TO RELEASE ACETYLCHOLINE, THE ENDOGENOUS TRANSMITTER THAT ACTIVATES NICOTINIC RECEPTORS Nicotinic Receptor Antagonist DHb. E Blocks Excitatory Effect of Released Acetylcholine

Ventral Lateral Geniculate Neuron (LGNv) ACh increases evoked glutamatergic currents by 3 -fold through activation of presynaptic nicotinic receptors

Schematic diagram of selected circuitry within the ventral Lateral Geniculate Nucleus (LGNv) a 4* a 6* a 7* a 6* Locations of nicotinic receptors are indicated by asterisks. Pharmacological tools allow identification of subtypes of n. ACh. Rs.

125 I-a-Conotoxin MII autoradiography in rat brain: LGN and optic tract heavily labeled revealing a 6* receptor locations A: Autoradiography of a 6* nicotinic receptors B and C: Confocal Microscopy showing Co-Localization of Synaptotagmin and a 7 nicotinic receptor subunit Co-localization (arrows) of a 7 n. ACh. R subunit and synaptotagmin in processes of an LGNv neuron Synaptotagmin (a presynaptic protein) immunolabeling showing synaptic terminals (white spots) around an SPL neuron (asterisk)