Verapamil Ameliorates Motor Neuron Degeneration and Improves Lifespan

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Verapamil Ameliorates Motor Neuron Degeneration and Improves Lifespan in the SOD 1 G 93

Verapamil Ameliorates Motor Neuron Degeneration and Improves Lifespan in the SOD 1 G 93 A Mouse Model of ALS by Enhancing Autophagic Flux Zhang Xiaojie, Chen Sheng, Lu Kaili, Wang Feng, Deng Jiangshan, Xu Zhouwei, Wang Xiuzhe, Zhou Qinming, Le Weidong, Zhao Yuwu 1 Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People&#x 00027; s Hospital, Shanghai, China. 2 Department of Neurology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. 3 Liaoning Provincial Center for Clinical Research on Neurological Diseases, the First Affiliated Hospital, Dalian Medical University, Dalian, China. 4 Liaoning Provincial Kay Laboratory for Research on the Pathogenic Mechanisms of Neurological Diseases, the First Affiliated Hospital, Dalian Medical University, Dalian, China. 5 Collaborative Innovation Center for Brain Science, the First Affiliated Hospital, Dalian Medical University, Dalian, China. Figure 5. Effects of verapamil on the numbers of autophagosomes and autolysosomes in the motor neurons of SOD 1 G 93 A mice. Representative EM photomicrographs of autophagosomes (marked by yellow arrowheads) and autolysosomes (marked by yellow arrows) in the motor neurons of WT-Vera mice (A) and Tg-Vera mice (B-D). (E) Quantitative analysis of the number of autophagosomes per field of 4 mouse groups. (F) Quantitative analysis of Aging and Disease, 2019, 10(6), 1159 -1173. DOI: 10. 14336/AD. 2019. 0228 autophagosome/autolysosome ratios per motor neuron of the 4 different mouse groups. Scale bar = 1 &#x 003 BC; m; n = 3 per group. Data were analyzed using one-way ANOVA followed by Tukey&#x 02019; s post hoc test. All values are presented as mean &#x 000 B 1; SEM. &#x 0002 A; P &#x 0003 C; 0. 01, compared with WT-Con group; &#x 00026; P &#x 0003 C; 0. 01 and &#x 00026; P &#x 0003 C; 0. 05, compared with Tg. Con group.