Variation of chemical composition of essential oils in

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Variation of chemical composition of essential oils in Thymus serpyllum L. subsp. serpyllum growing

Variation of chemical composition of essential oils in Thymus serpyllum L. subsp. serpyllum growing wild in Lithuania Kristina Ložienė Institute of Botany, Žaliųjų Ežerų g. 49, Vilnius, LT-08406, Lithuania (kristina. l@botanika. lt) Petras R. Venskutonis Department of Food Technology, Kaunas University of Technology, Radvilėnų pl. 19, Kaunas, LT-3028, Lithuania (rimas. venskutonis@ktu. lt)

 • The purpose of this study was to determine chemical composition of the

• The purpose of this study was to determine chemical composition of the oil of T. serpyllum subsp. serpyllum plants collected in various localities in Lithuania and to define the chemotypes within this subspecies growing wild in Lithuania.

 • Material and methods • In total 26 T. serpyllum subsp. serpyllum individual

• Material and methods • In total 26 T. serpyllum subsp. serpyllum individual plants (samples) were collected at the flowering stage in July from 14 different sites in Lithuania. • The oils were isolated from the selected individual plants by hydrodistillation in a Clevenger-type apparatus (European Pharmacopoeia) during two hours. GC and GC/MS carried out the analyses of the essential oils.

Collecting sites of Thymus serpyllum subsp. serpyllum samples

Collecting sites of Thymus serpyllum subsp. serpyllum samples

Range of the amount (%) of the main components essential oil in different Thymus

Range of the amount (%) of the main components essential oil in different Thymus serpyllum subsp. serpyllum samples

Two dimensional dendrogram obtained in the cluster analysis of the essential oils of samples

Two dimensional dendrogram obtained in the cluster analysis of the essential oils of samples of Thymus serpyllum subsp. serpyllum: (E)- -ocimene, -cadinol, cis-p-menth-2 -en-1 -ol, 1, 8 -cineole, germacrene B

 • This study proved that T. serpyllum subsp. serpyllum plants from the Northern

• This study proved that T. serpyllum subsp. serpyllum plants from the Northern climatic area don’t accumulate volatile phenolic compounds thymol and carvacrol in more remarkable amounts.

Thank you for your attention

Thank you for your attention