Varian 920 LC Integrated Analytical HPLC 1 Varian

Varian 920 -LC Integrated Analytical HPLC 1

Varian 920 -LC What Is It? • Compact, stylish automated analytical HPLC – Pre-plumbed and factory tested for the highest confidence – Single cable communications – Each system comes with solvent delivery system, autosampler and either UV Vis or PDA detector – Options of a second Fl or RI detector and column heater • Virtually pulse-free dual piston pump – Does not require a pulse damper • State-of-the-art low pressure quaternary gradient module with built-in degasser 2

Varian 920 -LC What Is It? • Autosampler with cooling option – Synchronised injection for outstanding retention time reproducibility – Compatible with 200 x 2 m. L HPLC vials or 3 microplates • Either sensitive dual l UV-Vis or PDA detector – Optional second detector - fluorescence or RI • High precision (<0. 15 °C) dual column heater available – Low delay solvent pre-heater for improved heat transfer – Optional column switching and electric injection valve • NEW improved instrument interface on Galaxie. TM software 3

Varian 920 -LC Silver Bullets • Fast LC with superb performance optimised for sub 3 mm columns – Improved throughput without compromising robustness • Pulse-free solvent delivery – No need for a pulse damper • High sensitivity detection • 4 Full system level computer controlled AIQ (validation)

80 Mobile Phase: Water Acetonitrile Gradient Detection: UV Detection Temperature: 60°C Flow-Rate: 1, 6 m. L/min Column: Varian Ultra 3 um 50 x 2, 0 mm 70 60 octanophenone 90 Conditions heptophenone 100 valerophenone m. AU Propiophenone 110 acetophenone Varian 920 -LC Fast LC Phenones 40 30 hexanophenone benzophenone 50 20 10 0 RT [min] 0. 1 5 0. 2 0. 3 0. 4 0. 5 0. 6 0. 7 0. 8 0. 9 1. 0 1. 1 1. 2 1. 3 1. 4 1. 5 1. 6 1. 7 1. 8

Varian 920 -LC Fast LC PAHs 6

Varian 920 -LC Fast LC Performance • Dual piston quaternary pump produces fast gradients – even at 200 m. L/min • Virtually pulse-free pumping without pulse damper • Outstanding gradient compositional accuracy give retention time precision at the <1% level • Built-in vacuum degas maintains pump prime and enhanced injection reliability • Overlay of 10 chromatograms • 10% steps at 1 m. L/min 7 • Accurate flow from 0. 1 to 10 m. L/min for 2 to 4. 6 mm columns

Varian 920 -LC Autosampler • Needle-in-flow path geometry for low carry-over (<0. 01%) • Typical inj precision (< 0. 3% at 10 m. L) • Optimized flow paths for fast injection turn-around • Synchronised injection for best RT reproducibility • Programmable wash setting for low carry-over • Injection volumes from 1 u. L to 50 m. L • Cooled option available 8

Varian 920 -LC Superb Washout 125 µg/m. L Caffeine Blank injection <0. 01% carryover 9

920 -LC Autosampler How Does it Work? 10

Varian 920 -LC Injection Synchronization 11

Sensitive UV-Vis Detectors Typical Agilent VWD S/N ~ 25 Typical Varian 920 -LC UV Vis S/N ~ 38 12

Varian 920 -LC Fluorescence Detector • Sensitive Fluorescence detector (S/N > 600 Raman H 2 O peak baseline method) • Time programmable l for increase sensitivity • Automatic l validation with built-in Hg lamp • Low 12 m. L dead volume flowcell for improved chromatography 13

Heated Column Compartment • Temperature range from ambient + 10 °C up to 65 °C – Temperature reproducibility +0. 15 °C – Ensures excellent analytical reproducibility – Uses a low delay volume pre-heater for efficient heat transfer to mobile phase – Spacious column compartment easily accommodates 2 x 250 mm columns – Option with electrically actuated column switching valve and injection valve 14

Outstanding Stability 15

System Reproducibility of 50 Injections Reproducibility <0. 8% over 50 injections and 12 hours 16

Varian 920 -LC Software Usability • User friendly Galaxie. TM software • Simplified, modern, consistent Instrument interface • Modular 11 pages • 920 -LC 4 pages!! • One page real-time status and chromatogram • Advanced integration and calibration • New IMS capabilities • Automated instrument validation • Reduces downtime and operating costs 17 • Fusion AE

Varian 920 -LC AIQ (Validation) AIQ (Analytical Instrument Qualification) • Fully PC controlled IQ and OQ (not PQ) • Puts the Varian 920 -LC at the forefront of regulated capabilities • Consistent, reproducible methodology • Includes pumps, autosampler, detectors, column heater • Chargeable option • Fully documented 18

Varian 920 -LC Ordering Matrix pump inject detector Modular Isocratic 210 -LC 10 m. L Manual 325 Isocratic 00 -100890 -00 Dual piston pump 10 m. L head Auto Dual l UV/Vis Opt FL, RI ELSD Gradient 00 -100891 -00 Quat, Grad, Degas Auto Dual l UV/Vis Opt FL, RI ELSD opt High Performance Gradient 00 -100892 -00 Quat, Grad, Degas Auto cooled Dual l UV/Vis Opt FL, RI ELSD opt Development 00 -100893 -00 Quat, Grad, Degas Auto PDA Std FL, RI ELSD opt Research 00 -100894 -00 Quat, Grad, Degas Auto cooled PDA Std FL, RI ELSD opt 19 Oven Detector 2 option Options PDA, RI, ELSD

Varian 920 -LC Target Markets • Strong historical position for Varian in analytical • Varian has the leading range of analytical technologies: spectroscopy, chromatography including consumables, data and services • Target Users • Environmental • Industrial • Generic Pharmaceutical * • Academia and Biotech • Same (but wider) target groups with a stronger product offering *40% of Regulated Pharma has significant competitive barriers due to installed software base 20

Varian 920 -LC Silver Bullets • Fast LC with superb performance optimised for sub 3 µm columns – Improved throughput without compromising robustness • Pulse-free solvent delivery – No need for a pulse damper • High sensitivity detection • 21 Full system level computer controlled AIQ (validation)

Varian 920 -LC Integrated Analytical HPLC 22