Varian 920 LC Competitors Varian 920 LC Market

Varian 920 -LC Competitors

Varian 920 -LC Market Competitors • Shimadzu • Waters • Agilent • Dionex • The 920 -LC presents a significant step forward for the Varian 2

Varian 920 -LC Compared to the Waters Alliance HPLC

Waters Alliance Consists of two versions • 2695 Separations Module • 2795 Separations Module • Pump, autosampler, column heater and controller • Pump features independent drive motors – Provides pulse-free operation w/o damper – Limited flow rate of 5 ml/min • 5 independent sample carousels – Sample capacity limited to 120 HPLC vials 4

Waters Alliance • Complete range of detectors • 2998 Diode Array Detector – Only 512 photodiodes – inferior resolution prone to interferences – Limited l range of 190 to 600 nm with D 2 lamp only • 2489 Dual wavelength Detector – Limited l range of 190 to 700 nm with D 2 lamp only • 2475 multi-wavelength Detector • 2414 RI Detector • 432 Conductivity Detector • 2420 ELS Detector • 2465 Electrochemical Detector 5

Varian 920 -LC Compared to Waters Alliance 6 Waters Alliance Varian 920 -LC Maximum flowrate 5 ml/min 10 ml/min Maximum pressure 5000 psi @ 3 ml/min 6000 psi @ 5 ml/min Autosampler Capacity 120 HPLC vials 200 HPLC vials Microplate Capability NA 3 plates Max Heater Temp 65 o. C 60 o. C Number of Detectors None Up to 2 built-in Diode Array 512 1024 UV-Vis l Range 190 - 700 190 - 900 Sample Carryover <0. 1% <0. 01%

Varian 920 -LC compared to the Agilent 1200 Series LC

Agilent 1200 Quaternary Pump G 1354 A • Dual piston, low pressure quaternary gradient pump • Electromechanical check valve • Variable piston stroke • Pulse damper between primary and secondary piston • Upgrade? ? of 1100 series • Requires degasser module 8

Agilent 1200 Autosamplers • Option of • G 1329 A standard Autosampler capable of 100 HPLC vials • G 1367 B Enhanced Autosampler compatible with either 2 microplates or 108 HPLC vials • Standard carryover <0. 1% but can be improved to <0. 01% using needle wash • Both have peltier cooled option 9

Agilent 1200 Detectors • Wide range of detectors • • • 10 Variable Wavelength Detector Multi-Wavelength Detectors Diode Array Detector Fluorescence Detector RI Detector

Sensitivity of 920 -LC UV-Vis vs Agilent 1200 Typical Agilent VWD S/N ~ 25 Typical Varian 920 -LC UV/Vis S/N > 36 11

Varian 920 -LC Compared to Agilent 1200 12 Agilent 1200 Varian 920 -LC Pulse Damper Yes Not Required Microplate compatibility Not Compatible Up to 3 96 well plates Autosampler Capacity 100 HPLC vials 200 HPLC vials Dual l UV-Vis NA Yes Carryover <0. 1% <0. 01% UV-Vis l range 190 – 600 D 2 only 190 – 900 D 2 & QI UV detector noise + 0. 75 x 10 -5 Au + 0. 5 x 10 -5 Au

Waters Purification Systems 13

Waters Delta 600 LC Analytical to Prep • Delta 600 wide flow rate range and 2 way injector valve allows operation at analytical and prep scales • Delta 600 LP – LP dual piston quaternary gradient pump • 0. 01 to 20 ml/min & up to 6000 psi • Up to 45 ml/min option • Stand alone 2 channel degasser option recommended 14

Prep. LC Dedicated Purification Systems • Prep. LC 150 and 300 – Dedicated preparative LC system quaternary gradient – 1 to 150 ml/min • Up to 4000 psi – 4 to 300 ml/min • Up to 2000 psi • Delta. Prep 150 and 300 – Same as Prep. LC but with solvent recycling capabilities 15

Waters Prep. LC 16 Sample Load Column ID Model Max Flow Rate (ml/min) mg – 10’s g 7. 8 – 75 mm Delta. Prep 300 ml/min mg – g 4. 6 – 50 mm Delta. Prep 150 ml/min mg – 10’s mg 4. 6 – 50 mm 2545 150 ml/min mg – 10’s mg 3. 9 – 30 mm Delta 600 EF 45 ml/min mg – 10’s mg 3. 9 – 19 mm 1525 + EF 22. 5 ml/min mg – 10’s mg 3. 9 – 19 mm Delta 600 20 ml/min mg - g 3. 9 – 7. 8 mm 1525 10 ml/min

Waters Detectors for Prep LC 17

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