Using Synthetic Biology and p Clone Red for
Using Synthetic Biology and p. Clone Red for Authentic Research on Promoter Function: Introductory Biology (identifying new promoters) A. Malcolm Campbell and Todd T. Eckdahl
GGA Method with p. Clone Red by A. Malcolm Campbell and Todd Eckdahl
Eco RI G AATTC CTTAA G type II palindrome
Eco RI G AATTC CTTAA G type II palindrome
Eco RI G AATTC CTTAA G type II
Eco RI G CTTAA AATTC G type II
Bsa I GAGAC C CTCTG G type IIs not a palindrome
Bsa I 1234 n. GAGAC C ---n. CTCTGG type IIs
Bsa I ---- 1234 n. GAGAC C n. CTCTGG type IIs
Bsa I GGTCTCn ---CCAGAGn 1234 type IIs
Bsa I GGTCTCn CCAGAGn 1234 type IIs ----
Bsa I cuts left 1234 n. GAGAC C ----n. CTCTGG GGTCTCn---CCAGAGn 123 4 cuts right
p. Clone Red = Part J 119137 Bsa. I cuts left Bsa. I cuts right
p. Clone Red Magnified GCG CGACt. GAGACC GGTCTCa GCT G a. CTCTGG CCAGAGt. CGCC G Bsa I
p. Clone Red Promoter Removed CGACt. GAGACC GGTCTCa a. CTCTGG CCAGAGt. CGCC GCT G GCG G
p. Clone Red Promoter Recloned GCG CGACt. GAGACC GGTCTCa GCT G a. CTCTGG CCAGAGt. CGCC G
p. Clone Red Promoter Removed Again CGACt. GAGACC GGTCTCa a. CTCTGG CCAGAGt. CGCC GCG G GCT G CGAC(promoter)CGCC
Experimental Promoter Cloned in p. Clone Re CGACt. GAGACC GGTCTCa a. CTCTGG CCAGAGt. CGCC CGAC(promoter) GCGG GCT (promoter)CGCC G
p. Clone Red in GGA GFP RBS Bsa. I + Ligase origin antibiotic resistance plasmid backbone RFP
p. Clone Red after GGA GFP RBS Bsa. I + Ligase origin antibiotic resistance plasmid backbone RFP
p. Clone Red + No Promoter p. Clone Red only notice only green colonies
p. Clone Red + Functional Promoter p. Clone Red notice red colonies
p. Clone Red + Non-functional Promoter X notice non-green colonies p. Clone Red
Registry of Standard Parts
Student Promoter (dna. KP 1)
Student Promoter (dna. KP 1)
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