Using a Microscope Carrying a Microscope 1 Make

Using a Microscope

Carrying a Microscope 1. Make sure cord is wrapped securely around the microscope 2. Carry the microscope with two hands – One hand on arm – One hand on base

Using the Microscope 1. Plug in microscope 2. Place the specimen on the stage 3. Make sure wet specimens have a coverslip (wet mount) – Protects objective lens from getting slimed 4. Turn on the light 5. Put the scanning objective in place 6. Using coarse adjustment, slowly bring the specimen into focus – Coarse adjustment is only used with the scanning objective

Use continued 7. Rotate the diaphragm to view the specimen in the best possible light 8. Move the slide, so that the object of interest is at the center of your field of view 9. If desired, turn the nosepiece so the next higher power objective is in place 10. Using the fine adjustment only, adjust the focus at this magnification. A correctly adjusted microscope will not hit the slide at high power 11. Repeat steps 8 -10 again to move to the next higher power

Cleaning the Microscope 1. Clean lenses using a soft cloth or lens paper 2. DO NOT use paper towel on lenses 3. Clean all other parts of the microscope with paper towels Do NOT Use on lens!

Storing the Microscope 1. Put the lowest power objective (scanning) in place 2. Using the coarse adjustment, push the nosepiece away from the stage (or the stage away from the nosepiece) 3. Turn off light 4. Unplug and wrap the cord around the microscope