Use of dialysis to separate small and large

Use of dialysis to separate small and large molecules

Gel filtration chromatography

Molecular mass determination by gel filtration chromatography

Some commonly used gel filtration materials

Ion-exchange chromatography with stepwise elution

Device for generating a linear concentration gradient

Molecular formulas of cellulose-based ion exchangers

Some biochemically useful ion exchangers

Affinity chromatography

Covalent linking of ligand to agarose

Derivatization of epoxy-activated agarose

Purification of staphylococcal nuclease by affinity chromatography on bisphothymidine-linked agarose

Experimental arrangement for paper chromatography

Two-dimensional paper chromatography

Paper electrophoresis

Apparatus for slab gel electrophoresis

Diagram of a disc electrophoresis apparatus

Polymerization of acrylamide and N, N¢-methylenebisacrylamide to form a cross-linked polyacryl-amide gel

SDS-PAGE

Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE

General formula of the ampholytes used in isoelectric focusing

Isoelectric points of several common proteins

Purification of rat liver glucokinase

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An enzyme-linked immunosorbent assay (ELISA)

Solubilities of several proteins in ammonium sulfate solutions

Solubility of caboxy-hemoglobin at is isoelectric point as a function of ionic strength and ion type

Solubility of b-lactoglobin as a function of p. H at several Na. Cl concentrations

Protein crystals

Agarose gel electrophoretogram of a double helical DNA

Pulsed-field gel electrophoresis (PFGE) of a set of different sized bacteriophage DNAs

Detection of proteins by immunoblotting

Two-dimensional (2 D) gel electrophoresis

Sedimentation coefficients in Svedbergs (S) for some biological materials

A selection of preparative ultracentrifuge rotors

Zonal ultracentrifugation

Equilibrium density distribution of a Cs. Cl solution in an ultracentrifuge spinning at 39, 460 rpm

Isopycnic ultracentrifugation

Physical constants of some proteins