UPConverting Phosphor Immunoassy Thechnology UpConverting Phosphor Quantitative Immunoassy
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UP-Converting Phosphor Immunoassy Thechnology Up-Converting Phosphor Quantitative Immunoassy Analyzer Bei. Jing Hotgen Biotech Co. , Ltd Jane Zhang
Company Profile Ø Beijing Hotgen Biotech Co. , Ltd was founded in 2005 and located in Biomedical Base of Zhongguancun High-Tech Park in Beijing. Ø Specializing in In-Vitro Diagnostic (IVD) Ø Point-Of-Care-Testing (POCT) Ø Biodefense Products Ø Raw Materials. www. hotgen. com. cn
The Development of Immunochromatographic Technology Two stage Visualization (Colloidal Gold immune test technology) Luminescence Four generation products CG Qualitative CG Semi-Quantitative Immunofluorescence Quantitative Up-converting Phosphor Quantitative
The Development of Immunochromatographic Technology First stage:Pigmentation particles(CG) The first generation product:CG Qualitative (HCG strip) Low sensitivity and less. Test specifity Visual inspection inaccuracy The. Test second generation product:CG Semi-quantitative results difficult to archive (color card or instrument)
The Development of Immunochromatographic Technology Second stage:Luminescent particles Luminescence background interference Third generation product:Fluorescence Quantitative Immunoassay analyzer Photobleach or Fluorescence fade Quantitative not accurate
The Development of Immunochromatographic Technology The Latest generation product Up-converting Phosphor(UCP)
2. UCP Technology Normal Fluorescent materials The luminescent material Up-converting Phosphor materials Artificially synthesized rare-earth-doped material, not exist in nature.
Up-converting phosphor particles: Rare-earth elements synthesized nonomaterials Rare-earth element (17): La、Ce、Pr、Nd、Pm、Sm、Eu、Gd、Tb、Dy、Ho、Er、 Tm、Yb、Lu;Sc and Y; UCPs: Usually submicron particles, made from rare-earth-doped ceramics and offen coated with silica for broader bio/chemical compatibility and ease of surface functionalization.
UCP Technology UCPs unique property: Optical up-conversion: The emission of light as a significantly shorter wavelength than that of excitation Exactly correspond with Anti-Stokes principle
UCP Technology Ø Stokes law (Stokes fluorescence): Ø Stokes fluorescence is the re-emission of longer wavelength photons (lower frequency or energy) by a molecule that has absorbed photons of shorter wavelengths (higher frequency or energy). Ø For example: Ultraviolet been excitation then re-emission the visible light. Ø Down-converting phosphors v Anti-Stokes fluorescence v Up-converting Phosphors
) ) Exciting light ( Emitting light ( Stokes Shift “Down-converting phosphor” Higher energy photon Exciting light ( Emitting light ( ) ) Ultraviolet light range UCPs Particles Zero optical background Lower energy photon Infrared range Visible light range Anti-Stokes Shift Unique“Up-converting phosphor” Higher energy photon Ultraviolet light range Fluorescence optical background interference Visible light range Lower energy photon Infrared range
UCP Technology UCP important characteristics ü Unique property of Up-conversion ü Zero optical background signal ü UCPs are photochemically stable do not photobleach or fade
UCP Technology A Perfect combination of UCP & Immunochromatographic Technology
Immunochromatographic lateral flow test strip Sample pad Preimmobilized antibody labeled UCP T LINE C LINE Before add sample PVC baseboard Connector pad membrane NC membranes Absorbent pad Reaction Complete reaction
UCP Technology v UPT & Immunochromatographic Technology Platform v UPT & Immunochromatographic test strip Research and Development v UPT & Immunochromatographic test strip Detection(measurement) Equipment
2. UCP Technology Platform Up-converting phosphor quantitative test kit (UPT test strip) Quantitative POCT Analyzer Independent Property Right UPT Test system Biosafety IVD POCT
The Quantitative POCT Analyzer Features Unique"Up-converting Phosphor" Technology Luminescence method/ Quantiative User-friendly operation easyer to control Repaid test 15 -20 mins can get result Compact design "mobile lab":ED clinical lab Highly reliable / accuracy of diagnosis for multidisease
UPT Analyzer operation precedure First step add sample Insert the complete reaction test strip into the UPT analyzer 20 -40 sec show's the accurate concentration Three steps repid test
Cardio-Vascular Disease Biomarker Test Items: c. Tn. I- (Tropionin-I) (Diagnosis of acute Myocardial infarction and risk stratification of ACS) H-FABP (The early diagnosis of AMI and ACS prognosis evaluation) NT-pro. BNP (The conventional indicator of HF diagnosis and treatment monitoring) CK-MB/MYO (The early stage biomarker of clinical diagnosis and prognosis evaluation of myocardial injury) www. hotgen. com. cn
Embolism Biomarker Test item: D-dimer Early stage exclusion and diagnosis indicator of the Deep vein thrombus Pulmonary embolism Suitable Department:Clinical lab. Department of Cardiology. ED. Oncology department etc www. hotgen. com. cn
Infection Diagnosis Biomarker Test Items: PCT-the标题 highest sentitivity the lowest detection limitation The high specific inficator of Bacterial infection diagnosis Differentitation and diagnosis of baterial & virus infection Guide resonable application of the Antibiotics CRP Highly accurate test for the detection of inflammation infection IL-6 The warning marker of early inflammation and sepsis occurrence Suitable Department:Pediatrics. ED. ICU. Pneumology dep. www. hotgen. com. cn
Rheumatoid Arthritis Diagnosis Product Test item: Anti-CCP The high sensitivity and specificity indicator of Rheumatoid Arthritis diagnosis www. hotgen. com. cn
Uniquely Recommended by China Association of Medical Equipment
Changzheng hospital clinical contrast report(Hotgen Vs Roche)
Changzheng hospital clinical contrast report(Hotgen Vs Roche)
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