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Making Molecular Movies with Your Name, Institution

The Team University of Georgia Jessica Winter Chemical Engineer Ge Yang Bioengineer Peter Kner Microscopist Beth Brainerd Biologist

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Visit us online! qstorm. org Time is ticking. Can these folks make a major breakthrough in biological imaging?

48 hours in 40 seconds Insert video: Time. Lapse. Construction. m 4 v Credit: www. broad. com; The Ark Hotel

10 microns Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center

10 microns Insert video: Cell. Division-Short. m 4 v Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center

10 microns Insert video: Cell. Division-Short. m 4 v Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center

10 microns Insert video: Cell. Division-Short. m 4 v Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center

10 microns Insert video: Cell. Division-Short. m 4 v Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center

The Team University of Georgia Jessica Winter Chemical Engineer Ge Yang Bioengineer Peter Kner Microscopist Beth Brainerd Biologist

Beth Brainerd Biologist (Muscle Cells)

Zebrafish Muscle Cells Insert video: loop-and-slow-motion-female-sp_prores. mov Credit: Mezzotint

Ge Yang Bioengineer (Nerve Cells)

Nerve Cells Credit: “Inside the Brain”, NIH National Institute on Aging

Insert video: Inner. Life. Trimmed. m 4 v “Inner Life of the Cell”, Harvard University © 2008

≈ 100 nanometers “Inner Life of the Cell”, Harvard University © 2008

≈ 100 nanometers “Inner Life of the Cell”, Harvard University © 2008

Motor Protein

Much smaller than a wavelength of visible light 30 nm

Point light source 30 nm

Optical Limit: 200 nanometers 200 nm

Overlapping Blurs 200 nm

The Team University of Georgia Jessica Winter Chemical Engineer Ge Yang Bioengineer Peter Kner Microscopist Beth Brainerd Biologist

Peter Kner Microscopist

STORM Insert video: massive-electrical-storm-light_prores. m 4 v Insert sound: Perfect Thunder Storm-Sound. mp 3 Credit: Moving. Images

STORM IMAGING

STORM STochastic Optical Reconstruction Microscopy

STORM Xiaowei Zhuang 2006 Credit: Fred. Field. com

Standard optical microscope image 3 μm Credit: Bates et al. Science 317, 1749 -1753 (2007)

Loss of resolution when zoomed into the nanoscale Credit: Bates et al. Science 317, 1749 -1753 (2007)

Standard Microscope Image Diffraction-Limited STORM Image Super Resolution Credit: Bates et al. Science 317, 1749 -1753 (2007)

+ + + Localization + +

Insert video: Particle Detection. m 4 v

Greetings from Insert video: Reconstructed Image. m 4 v

STORM Imaging at Work Insert video: Particle Detection. m 4 v (on left side) and Reconstructed Image. m 4 v (on right side) Stochastic (Random) Blinking Reconstruction Credit: Ricardo Henriques, Institut Pasteur

STORM Imaging at Work Insert video: Particle Detection. m 4 v (on left side) and Reconstructed Image. m 4 v (on right side) Particle Detection and Localization Reconstruction Credit: Ricardo Henriques, Institut Pasteur

STORM Imaging of Cell Structures Insert video: Zhuang_storm_short_slow. avi Credit: Rust et al. Nature Methods 3, 793 -795 (2006) and Bates et al. Science 317, 1749 -1753 (2007)

STORM – Depends on bright light sources Credit: Bates et al. Science 317, 1749 -1753 (2007)

STORM – Depends on bright light sources Less than 30 nm ! Credit: Bates et al. Science 317, 1749 -1753 (2007)

STORM – How to Get Even Greater Resolution? Less than 30 nm ! We need more photons! Credit: Bates et al. Science 317, 1749 -1753 (2007)

The Team University of Georgia Jessica Winter Chemical Engineer Ge Yang Bioengineer Peter Kner Microscopist Beth Brainerd Biologist

Jessica Winter Chemical Engineer

Quantum Dots in Solution Nano Crystals of Different Sizes Credit: Felice Frankel

Jessica Winter Chemical Engineer

Insert video: Particle Detection. m 4 v Credit: Ricardo Henriques

10 nm

Linker

Super. Heroes

Insert video: vim-NEi. A_Fall 2010_Mo. S_QSTORM_Final. mov Or download or play from: http: //vimeo. com/18462954

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Making Molecular Movies with An MOS Strategic Projects Production by C. L. Alpert and K. Thate with assistance from J. Antill and the QSTORM Research Collaborative Production of this project was supported by the National Science Foundation, MCB-1052733. Dissemination of this project was supported by the NISE Network through the National Science Foundation under Award No. 0940143. Any opinions, findings, and conclusions or recommendations are those of the author(s) and do not necessarily reflect the views of the Foundation. Published under a Creative Commons Attribution-Noncommercial. Share. Alike license: http: //creativecommons. org/licenses/by-nc-sa/3. 0/us Join us at www. QSTORM. org