UE Metabolomics Ass Student Mara Vallianou Gert Bachmann

UE Metabolomics Ass. Student: Mara Vallianou Gert Bachmann Team Lena Fragner Ass. Student: Martin Brenner Stefanie Wienkoop Wolfram Weckwerth Thanks to the Green Team Andi und Thomas ! Dept. Molecular Systems Biology University Vienna for private use only!

Stefanie Wienkoop - Metabolomics Wednesday Thursday Friday 05. 2. 2020 06. 2. 2020 07. 2. Intro & GLP * plant harvesting LAB work short intro * metabolite extraction *derivatisation Monday Tuesday 10. 2. 11. 2. Theory Software, MA data analysis LAB work short intro Theory *Experimental background Plants *Systems Biology Saturday/ Sunday Wednesday 12. 2. MS measurement (no regular course program) Statistical Analysis & & Interpretation of Results Theory *GC-MS *sample loading Dept. Molecular Systems Biology University Vienna for private use only!

Experimental Setup Stefanie Wienkoop - Metabolomics Clusia spp. : (8) mayor (rosea) alata hilariana Clusia major minor valeri multiflora mexicana amazonica Leaf samples! ? University Vienna for private use only!

Stefanie Wienkoop - Metabolomics Experimental Setup Group 1 mayor, multiflora, amazonica, minor each group - 4 Clusa spp. & - 3 replicates Clusia major Group 2 alata, hilariana, minor, mexicana Group 3 hilariana, valerii, amazonica, mexicana Group 4 mayor, alata, valerii, multiflora University Vienna for private use only!

Stefanie Wienkoop - Metabolomics Full Scientific Experiment ! Harvest Extraction Analytics Statistics University Vienna for private use only!

Stefanie Wienkoop - Metabolomics Scientific Question MS-Techniques 1) Metabolite Extraction 2) GC-Triple quadrupole MS (Metabolite profiling and quantitative analysis) 3) Data Mining – Statistical Analyses Tools (Multivariate) Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!

Metabolomics – Technical Strategies University Vienna - Stefanie Wienkoop – Plant Systems Interaction Part I Technical Strategies Complementary and Integrative, quantitative Proteomics and Metabolomics MS Analysis techniques in Systems Biology GC-Triple. Quadrupole. MS GCx. GC-Tof MS TARGETED LC-Triple. Quadrupole. MS UNBIASED LCOrbitrap. MS Dept. Molecular Systems Biology for private use only!

Stefanie Wienkoop - PPMetabolomics 4 Groups á 2 -3 persons Dept. Molecular Systems Biology University Vienna for private use only!

Metabolomics Stefanie Wienkoop - PPMetabolomics What do we expect from you? 1) Excellent collaboration 20% 2) Final presentation of your results in english: a) Protocol (in groups, written in english, article formate) please label your paragraphs with Author names! 40% b) Oral presentation (date according to prior agreement) 40% Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!

Metabolomics Stefanie Wienkoop - PPMetabolomics Protocol in groups! In publication similar style! Introduction with literatur research, M+M in Protocol style (xµL pipetted. . ) Discussion Deadline: February 29 th ! Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!

Metabolomics ecology learning Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!

Metabolomics Login: User: pdfuser Password: pdfpass Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!

UE Metabolomics 4 Groups of 3 Students Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!