UE Metabolomics Ass Student Mara Vallianou Gert Bachmann
UE Metabolomics Ass. Student: Mara Vallianou Gert Bachmann Team Lena Fragner Ass. Student: Martin Brenner Stefanie Wienkoop Wolfram Weckwerth Thanks to the Green Team Andi und Thomas ! Dept. Molecular Systems Biology University Vienna for private use only!
Stefanie Wienkoop - Metabolomics Wednesday Thursday Friday 05. 2. 2020 06. 2. 2020 07. 2. Intro & GLP LAB work * plant harvesting short intro * metabolite extraction *derivatisation Saturday/ Sunday Theory *GC-MS Tuesday 10. 2. 11. 2. Wednesday 12. 2. Theory Software, MA data analysis MS measurement (no regular course program) Theory *Experimental background Plants *Systems Biology Monday Statistical Analysis & & Interpretation of Results Theory *sample loading Dept. Molecular Systems Biology University Vienna for private use only!
Experimental Setup Stefanie Wienkoop - Metabolomics Clusia major Clusia spp. : (8) mayor (rosea) alata hilariana minor valeri multiflora mexicana amazonica ? Leaf samples! University Vienna for private use only!
Stefanie Wienkoop - Metabolomics Experimental Setup Group 1 major, multiflora, amazonica, minor each group - 4 Clusa spp. & - 3 replicates Clusia major Group 2 alata, hilariana, minor, mexicana Group 3 hilariana, valerii, amazonica, mexicana Group 4 major, alata, valerii, multiflora University Vienna for private use only!
Stefanie Wienkoop - Metabolomics Full Scientific Experiment ! Harvest Extraction Analytics Statistics University Vienna for private use only!
Stefanie Wienkoop - Metabolomics Scientific Question MS-Techniques 1) Metabolite Extraction 2) GC-TOF MS (Metabolite profiling, identification and quantitative analysis) 3) Data Mining – Statistical Analyses Tools (Multivariate) Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!
Metabolomics – Technical Strategies University Vienna - Stefanie Wienkoop – Plant Systems Interaction Part I Technical Strategies Complementary and Integrative, quantitative Proteomics and Metabolomics MS Analysis techniques in Systems Biology GC-Triple. Quadrupole. MS GCx. GC-Tof MS TARGETED LC-Triple. Quadrupole. MS UNBIASED LCOrbitrap. MS Dept. Molecular Systems Biology for private use only!
Stefanie Wienkoop - PPMetabolomics 4 Groups á 2 -3 persons Dept. Molecular Systems Biology University Vienna for private use only!
Metabolomics Stefanie Wienkoop - PPMetabolomics What do we expect from you? 1) Excellent collaboration 20% 2) Final presentation of your results in english: a) Protocol (in groups, written in english, article formate) please label your paragraphs with Author names! 40% b) Oral presentation (date according to prior agreement) 40% Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!
Metabolomics Stefanie Wienkoop - PPMetabolomics Protocol in groups! In publication similar style! Introduction with literatur research, M+M in Protocol style (xµL pipetted. . ) Discussion Deadline: February 29 th ! Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!
Metabolomics ecology learning Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!
Metabolomics Login: User: pdfuser Password: pdfpass Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!
UE Metabolomics ? ? ? Any questions so far? ? ? Let‘s start!!! Discuss Sample names 4 Groups of 3 Students Today morning • • Labeling of tubes Seminar room Safety Introduction – GLP LAB Harvest Green house Grinding and weighing Stefanie Wienkoop – Plant Systems Interaction Dept. Molecular Systems Biology University Vienna for private use only!
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