Tubular Organs Tracheal Development DB DT LT Dysfusion

Tubular Organs

Tracheal Development DB DT LT

Dysfusion (Dys) is a b. HLH-PAS Protein b. HLH PAS-1 PAS-2

Dysfusion Is Expressed In Fusion Cells DB DT LT Btl-GAL 4, UAS-lac. Z Dys

2 3 dys dys Mutants b. HLH PAS-1 PAS-2 WT dys Mab 2 A 12

How dys expression is regulated? How Dys: Tgo regulates down-stream target genes?

Dysfusion is Expressed in Mutiple Cell Types

Identify dys Enhancer

Identify dys Fusion Cell Enhancer

Ongoing…… How dys expression is regulated? • Is dys auto-regulated by Dys: Tgo? N 283 -GFP, dys/Tm 3, twi-gal 4, UAS-GFP C 1. 7 -GFP, dys/TM 3, twi-gal 4, UAS-GFP • Is dys regulated by esg? esg/cyo, twi-gal 4, UAS-GFP; N 283 esg/cyo, twi-gal 4, UAS-GFP; C 1. 7 • Identify other transcription factors binding sites in dys enhancer. Identify dys fusion cells enhancer in C 1. 7 and G 1. 7

Summary of dys Function Dys Tgo N Identified dys Targets Dys Tgo TC GTG CG 13196, E-cadherin (cell adhesion) CG 15252 (unknown function) members only (Protein trafficking) Trachealess (Migration)

Two TCGTG Sites are Required to Activates CG 13196

Two TCGTG Sites of CG 13196 are Conserved

Two TCGTG Sites are Required to Activates CG 15252

Two TCGTG Sites of CG 15252 are Conserved

Potential Co-activator of Dys: Tgo is Required to Turn on Gene Expression in Additional Fusion Cells

Ongoing. .