TORTORA FUNKE CASE Microbiology AN INTRODUCTION EIGHTH EDITION
TORTORA • FUNKE • CASE Microbiology AN INTRODUCTION EIGHTH EDITION B. E Pruitt & Jane J. Stein Chapter 9, part B Biotechnology and Recombinant DNA Power. Point® Lecture Slide Presentation prepared by Christine L. Case Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings
Genetic Engineering Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 11. 1
Genetic Engineering Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 11. 2
Making a Gene Product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 12. 1
Making a Gene Product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 12. 2
E. coli • Used because it is easily grown and its genomics are known • Need to eliminate endotoxin from products • Cells must be lysed to get product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings
Making a Gene Product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 13
Therapeutic Applications • Subunit vaccines • Nonpathogenic viruses carrying genes for pathogen's antigens as vaccines • Gene therapy to replace defective or missing genes • Human Genome Project • Nucleotides have been sequenced • Human Proteome Project may provide diagnostics and treatments Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings
Random Shotgun Sequencing Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 14
Scientific Applications • Understanding of DNA • Sequencing organisms' genomes • DNA fingerprinting for identification Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 16
Southern Blotting Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 15. 1
Southern Blotting Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 15. 2
Southern Blotting Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 15. 3
Agricultural Applications Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Table 9. 2
Genetic Engineering Using Agrobacterium Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 18
Safety Issues and Ethics • Avoid accidental release • Genetically modified crops must be safe for consumption and for the environment • Who will have access to an individual's genetic information? Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings
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