TORTORA FUNKE CASE Microbiology AN INTRODUCTION EIGHTH EDITION

TORTORA • FUNKE • CASE Microbiology AN INTRODUCTION EIGHTH EDITION B. E Pruitt & Jane J. Stein Chapter 9, part B Biotechnology and Recombinant DNA Power. Point® Lecture Slide Presentation prepared by Christine L. Case Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings

Genetic Engineering Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 11. 1

Genetic Engineering Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 11. 2

Making a Gene Product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 12. 1

Making a Gene Product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 12. 2

E. coli • Used because it is easily grown and its genomics are known • Need to eliminate endotoxin from products • Cells must be lysed to get product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings

Making a Gene Product Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 13

Therapeutic Applications • Subunit vaccines • Nonpathogenic viruses carrying genes for pathogen's antigens as vaccines • Gene therapy to replace defective or missing genes • Human Genome Project • Nucleotides have been sequenced • Human Proteome Project may provide diagnostics and treatments Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings

Random Shotgun Sequencing Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 14

Scientific Applications • Understanding of DNA • Sequencing organisms' genomes • DNA fingerprinting for identification Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 16

Southern Blotting Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 15. 1

Southern Blotting Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 15. 2

Southern Blotting Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 15. 3

Agricultural Applications Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Table 9. 2

Genetic Engineering Using Agrobacterium Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 9. 18

Safety Issues and Ethics • Avoid accidental release • Genetically modified crops must be safe for consumption and for the environment • Who will have access to an individual's genetic information? Copyright © 2004 Pearson Education, Inc. , publishing as Benjamin Cummings