TOPIC Microscope AIM How do we use a

  • Slides: 26
Download presentation
TOPIC: Microscope AIM: How do we use a microscope?

TOPIC: Microscope AIM: How do we use a microscope?

Total magnification • Eyepiece lens magnification x objective lens magnification

Total magnification • Eyepiece lens magnification x objective lens magnification

Total magnification=4 x 10= 40

Total magnification=4 x 10= 40

100 x 400 x

100 x 400 x

Rules • 1. Carrying microscope = when • 1 hand on arm, other hand

Rules • 1. Carrying microscope = when • 1 hand on arm, other hand under base close to using a microscope body

 • 2. DO NOT use coarse adjustment under high power • bc it

• 2. DO NOT use coarse adjustment under high power • bc it can break the lens or the slide

 • 3. Always start off with low power bc it gives you a

• 3. Always start off with low power bc it gives you a greater field of view. LOW HIGH

 • 4. Before switching to high power you must: • a. focus your

• 4. Before switching to high power you must: • a. focus your image • b. center your image

 • 5. Adjust the diaphragm • (When switching from low to high, amount

• 5. Adjust the diaphragm • (When switching from low to high, amount of light decreases)

 • 6. Use LENS PAPER to clean lens

• 6. Use LENS PAPER to clean lens

What is • Treating staining? specimen with solutions of different colors • Make cell

What is • Treating staining? specimen with solutions of different colors • Make cell parts more visible

Unstained cheek cells Stained cheek cells

Unstained cheek cells Stained cheek cells

 • Examples: • 1. Lugol’s Iodine • (plant cells)

• Examples: • 1. Lugol’s Iodine • (plant cells)

Onion Cells stained with Lugol’s Iodine

Onion Cells stained with Lugol’s Iodine

 • 2. Methylene blue • (animal cells)

• 2. Methylene blue • (animal cells)

Cheek Cells Unstained Stained

Cheek Cells Unstained Stained

Skin Cells stained with Methylene Blue

Skin Cells stained with Methylene Blue

Wet • 1. Place a drop of water on the slide mount

Wet • 1. Place a drop of water on the slide mount

 • 2. Place object on the slide.

• 2. Place object on the slide.

 • 3. Slowly lower cover slip onto specimen on an angle to avoid

• 3. Slowly lower cover slip onto specimen on an angle to avoid AIR BUBBLES.

 • 4. Absorbed excess water with a paper towel.

• 4. Absorbed excess water with a paper towel.

How do • Upside down & backwards images appear? On the slide Seen through

How do • Upside down & backwards images appear? On the slide Seen through lens F

What • Image moves in happens opposite direction when you move the slide?

What • Image moves in happens opposite direction when you move the slide?