TOPIC Microscope AIM How do we use a
- Slides: 26
TOPIC: Microscope AIM: How do we use a microscope?
Total magnification • Eyepiece lens magnification x objective lens magnification
Total magnification=4 x 10= 40
100 x 400 x
Rules • 1. Carrying microscope = when • 1 hand on arm, other hand under base close to using a microscope body
• 2. DO NOT use coarse adjustment under high power • bc it can break the lens or the slide
• 3. Always start off with low power bc it gives you a greater field of view. LOW HIGH
• 4. Before switching to high power you must: • a. focus your image • b. center your image
• 5. Adjust the diaphragm • (When switching from low to high, amount of light decreases)
• 6. Use LENS PAPER to clean lens
What is • Treating staining? specimen with solutions of different colors • Make cell parts more visible
Unstained cheek cells Stained cheek cells
• Examples: • 1. Lugol’s Iodine • (plant cells)
Onion Cells stained with Lugol’s Iodine
• 2. Methylene blue • (animal cells)
Cheek Cells Unstained Stained
Skin Cells stained with Methylene Blue
Wet • 1. Place a drop of water on the slide mount
• 2. Place object on the slide.
• 3. Slowly lower cover slip onto specimen on an angle to avoid AIR BUBBLES.
• 4. Absorbed excess water with a paper towel.
How do • Upside down & backwards images appear? On the slide Seen through lens F
What • Image moves in happens opposite direction when you move the slide?
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- Microscope mania compound light microscope
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- Who discovered the cell
- Care and structure of the compound microscope
- Compound microscope use
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