To Drink or not to Drink By Lauren

To Drink or not to Drink? By Lauren Morris

My name is Lauren Morris. I am a homeschooled 5/6 th grader, and this is my first year taking FLVS classes. I just finished M/J Science 1, Career Education, and Art 2 -D. I am currently finishing M/J Language Arts 1 and just started Spanish. This the first opportunity I have had to participate in a school science fair. I have done several long term science projects through 4 H including Butterfly Wings, Magic of Electricity, and Marine Science. In 2010 I won a blue ribbon and a big Trophy for 2010 Best of Show Exhibit for Science and Technology for my electricity project. I also won a blue ribbon and a trophy for the 2010 4 H Photo Contest, Digital Single Print, Junior Division for my nature photo “Sand Dune Sunset”, which also advanced to state. I also won the Seminole County Medal for Science and Technology Achievement that year. I had a lot of fun participating in the 4 H Florida State Marine Ecology Competition the following year. This year I attended Project Create with my Gifted class, where we presented our group project on the prosthetic voice, and I built an unbreakable bridge! I just got the results back and I won the Blue Award for my praying mantis board this year. Currently I am working on an entomology project. I have loved science, math, and art since I was really little. While I enjoy chemistry, physics and geology, biology is my real favorite. I’m always collecting specimens, studying new ideas, building stuff, or experimenting with something to do with science. About me

The Importance of my Project Water is the most important thing in the world! Water covers 70% of all earth, but only 1% of all water on earth is useable by humans. Water is the source of all life and is needed by every living creature and plant. Monitoring the quality of water can tell us how healthy our environment is. We all need to take responsibility to care for our local bodies of water, because there is only a limited supply for all people, animals, and plants to use. The consequences of poor water quality could not only harm wildlife, but humans as well. Florida is proud of its clean environment, but the government cannot constantly monitor all natural areas for problems. Checking these areas shows where the water, or the environment around it, might need cleaning. This is just doing our part to take care of our earth.

Hypothesis Question: Is our Central Florida surface water a safe habitat for animals to live in and drink? Hypothesis: The quality of surface water in Central Florida varies depending on the habitats and human impact. Variables: habitats where I took the samples, watersheds, weather, salinity levels, and water depth Constants: the test kit tablets, water quantity collected

Initial Research: How I Choose My Theme I love animals and wanted to help do something for them as part of my project. My Girl Scout troop visited a water treatment facility in Ormond Beach, and we learned all about how our public water supply was treated through Osmosis. They taught us all about the chemistry behind water testing. They even let us do some of the experiments, and I got to perform one of the tests. I wanted to learn more about this, so that is why I choose to focus on water quality. Doing further research, I found the World Water Monitoring Website and learned that nobody had tested in Florida, and I wanted to be the first. We ordered the kit and adapted their charts to include more important environmental information.

Initial Research: Works Cited WWMD Lesson Plans: http: //www. worldwatermonitoringday. org/Guides_Lesson_Plans. aspx WWMD Guides: http: //www. worldwatermonitoringday. org/Observation_Guide. aspx “Watershed Action Volunteers” Pamphlet, St. Johns River Management District, 2011 “How You Can Protect Central Florida Waterways”, Pamphlet, Seminole County Florida’s Natural Choice, 2011 Environmental Protection Agency website www. epa. gov Florida Department of Environmental Protection http: //www. dep. state. fl. us/watersheds/

Research Tests v Turbidity is how cloudy the water sample is. All surface water contains some solid matter, which can be of different sizes. Some of the solids might be salts, detritus, animal waste, dirt, sand, leaves, sticks, micro-organisms, bacteria, amoebas, and chemicals from the surrounding environment, such as run off fertilizers, natural tannin from cypress trees, limestone from a spring, or oil from nearby roads. Human garbage can also cause increased turbidity. The small solid particles cause the water to look more turbid, or cloudy. The less turbid the water, the healthier the environment. Turbidity is a key test of water quality, because sunlight has to pass through it for the plants in the water to grow well through photosynthesis. The solids also cause the water to be warmer, because they trap and absorb heat energy. Turbid water it is often darker, too, and darker colors absorb more heat. Increased turbidity also slows the production of dissolved oxygen (DO), because the plants give off oxygen during photosynthesis. v p. H is how acidic or basic the water is. The p. H scale is 1 to 14. Pure water is neutral, with a p. H of 7. Water that has a p. H less than 7 is acidic, and greater than 7 is basic, or alkaline. Most wildlife thrives in a p. H of 6. 5 to 8. 0. Outside that range animals might get sick, die or leave the area. Environment factors that affect p. H include acid rain, waste water, runoff, groundwater minerals, mining, and trees such as Cypresses that give off tannin.

Dissolved Oxygen(DO) measures how many singular oxygen molecules are in the water, beyond the H 2 O. Oxygen is an important part of respiration for fish and other aquatic animals. Plants make the oxygen in the water through photosynthesis. Higher DO readings mean more species of creatures can live in it, and the water will be healthier. Low levels are bad and can weaken or kill fish and other aquatic life. Bacteria and plant decomposition cause saturation levels to go down. This can also be caused by trash, silt, and algae blooms. Temperature is important to water quality, too. For each site, I measured both air temperature and water temperature where the sample was collected. We needed to use Celsius because it is the scientific measure of temperature. Temperature readings are important because they effect DO, rate of photosynthesis, and the food supply for aquatic animals. Waters too hot, or too cold, can have very harmful affects on fish and other cold blood animals. Vegetation such as trees provide shade to keep ponds and steams more regulated during Florida’s hot summers. Run off from hot black top roads makes a difference too. These factors all change the ecosystem. Image Credit: http: //islandwood. org/kids/stream_health/Data/DO_Ranges. html

Materials DO tablets Tes-Tab (3976) PH tablets – Wide Range Tes. Tab (6459) Color Coded Comparison Charts Site Data/Research Charts Thermometer Collection Container Two test vials - one big, one small Turbidity Wheel

How I did it 1. I selected several different types of habitats common in Central Florida. 2. From each location, I used a clean collection container, rinsed it with the water at the location, and then faced it in the direction of the water flow (if present) for 30 seconds before collecting the final sample and capping the container. 3. I then measured the depth of the water where the sample was collected. 4. Two vials were submerged into the collection container and filled immediately. 5. In the smaller vial, I inserted two DO tablets and sealed it tightly, making sure no air bubbles were in the vial. After the tablets dissolved, I compared the vial’s color against the DO color chart and recorded the results. 6. In the large vial, I collected 10 ml of water and inserted one p. H tablet. Once this tablet dissolved, I compared its color against the p. H color chart and record the results. 7. I then recorded the air temperature and the temperature of the water at the depth it was collected. 8. For each habitat, I recorded any environmental factors which might influence the water quality. 9. Then I had to dilute the samples with a large amount of water before it could be dumped back in the water. 10. Lastly, each vial had to be cleaned and stored for the next use.

Data Collection Method My data was collected on paper charts which was then transferred into an MS Excel Spreadsheet. Those records are included in this Power. Point as the following tables. I collected data using the World Water Monitoring Day Water Quality Kit equipment and also recorded additional environmental factors like land use, human water use, the source of origin, shoreline plant life, air and water temperatures, weather factors, visual clarity, macro-biologicals, water depth at the sample location, signs of erosion and natural debris.

Collection Locations

Table: Upper East Coast Basin Location/Factors Land use Human water use Source Shoreline plantlife Trees Shrubs Grasses Air temperature Water temp Weather today Weather yesterday Visual check Insects present Identify Macro-Invertebrates Identify Wildlife Identify Depth of water Erosion present Describe/ causes Natural debris Identify p. H DO % saturation Turbidity Halifax River Atlantic Ocean Renick Pk, Ormond mixed use Hotels Wading, fishing, small Kayaking, fishing, craft, large boats swimming Atlantic ocean Atlantic Yes Palm trees Small bushes none River grasses Dune grasses, sea oats 26°c 24°c 22°c sunny windy sunny partly sunny Trash, foamy, clear yes No Bees, flies n/a Yes yes Snails, mussels, crabs Sand fleas, shrimp Yes yes Mullet, yellowtail jack birds, fish catfish, minnow 2 feet No No N/A N/a Yes Rocks, shells, oysters Shells, seaweed 7. 5 95 92 70 JTU 40 JTU Residential Water Holly Hill houses Drinking, dishwashing, bathing, laundry, etc. City water plant No No 26°c 24°c Sunny clear No N/A No n/a Lake Hadley Canal Holly Hill park/roads Fishing Drainage water Yes Palm, oak, sweet gum Small bushes St. Augustine 24°c 26°c Partly sunny Cloudy Trash, murky Yes water striders No N/A Yes Birds, fish Directly out of tap N/A N/a No n/a 7. 5 48 0 JTU 4 inches Yes Water flowing Yes Sticks, logs, algae 7. 5 99 40 JTU Halifax River Marsh mixed use Boating, fishing, wading Halifax River Yes Palms, oaks Cedar Misc. shore grasses 27°c 24°c sunny Trash , murky yes water striders, bees flies no N/A yes Ducks, crows, seagulls, fish 6 inches No N/A Yes Rocks, sticks, branches 7. 5 76 60 JTU

Table: Middle St Johns’ Watershed Location/Factors Question Pond Spring Hammock Preserve Longwood Soldier Creek Spring Hammock Preserve Untreated Water from a Residential Artesian Well Oviedo Land use Human water use Source Shoreline plant life Trees Shrubs Grasses Air temperature Water temp Weather today Weather yesterday Visual check Insects present Identify Macro-Invertebrates Identify Wildlife Identify Education No Spring yes Palm, elm, tupelo bushes Cabbage, ivy, ferns 24°c 22°c sunny partly sunny, rainy AM Trash, clear, milky Yes water striders, flies , spiders, butterflies Yes crayfish/leeches Yes birds /minnows Education No Question pond spring Yes Tupelo small bushes water plants 27°c 20°c sunny partly sunny, rainy AM Trash, clear Yes flies, water striders, dragonflies None N/A Yes birds/butterflies/minnows Houses Pond the aquifer Yes Oak, laurel none St. Augustine 26°c 24°c sunny partly sunny clear Yes mosquitos None N/A yes Depth of water Erosion present Describe/ causes Natural debris Identify p. H DO % Saturation Turbidity 8 inches yes runoff/protected by roots yes Sticks, logs, leaves 8 92 0 JTU 3 inches Yes nature water run off Yes Sticks, leaves 6. 5 88 40 JTU N/A none N/A yes leaves 8. 5 24 0 JTU birds

lif Ha g e -Pk , GA . l. . Ho l . . ll Hi . . . B. Ho ly Ho l d on rm nr ise sia. . . Pr es te Ar Su of LP ity un ris -S of Pk O -C on al sh ar na l Ca at er l. W r. M Ri ve ey Ha dl ax ke tia ick e tia l en or Sh -R en th or id Turbidity (JTU) La ea n Oc en sid Re ic nt r, N . . . re Un 0 ve Ri oc k m k. P oc m Ha m er , R es at W g g in pr , S 20 At la ax lif Ha pr pr in ek , S re , S nd Po ed at re Un t r. C ie ld So io n st Qu e Po nd ee Cr n 8, 5 in g k, Ha tr Sp ea m rin m te oc g d Ha W Ha k. P a m lif re te m ax s. . r, oc Re. Ri k. P ve sid At r, re la en No nt se tia ic r. . r th l. A Oc. Sh rt ea e or sia n e -R Re n of e. . . sid ni S un ck e nt La ris Pk ial ke e O P. Ha W rm. . at dl o er nd ey Ha -C Ca Be lif ity na ax a. . . of la Ri lo ve Ho n r. M lly g LP Hi ar G ll sh A -S -Ho un lly ris e. . . Pk , H ol ly. . . er ld i So tio es Qu DO – Turbidity Correlation Graphs 120 100 80 60 40 DO % Saturation p. H 9 8 7, 5 7 6, 5 6 p. H

100 No obvious DO – Temperature Correlation 95 90 85 80 75 70 65 60 55 Air temperature °c 50 Water temp °c 45 DO % Saturation 40 35 30 25 20 Question Soldier Creek, Untreated Halifax River, Atlantic Ocean Residential Lake Hadley Halifax River Pond, Spring Water, North Shore of - Renick Pk Water - City of Canal along Marsh - Sunrise Hammock Residential Sunrise Pk - Ormond Beach Holly Hill LPGA -- Holly Pk, Holly Hill Preserve, Artesian Well, Holly Hill Longwood Black Hammock, Oviedo

Discussion You can see a correlation on the DO/turbidity chart where they follow the same pattern of ups and downs. This makes sense because increased turbidity would slow photosynthesis, because it blocks the sunlight that the plants need. The plants produce the oxygen. On the other hand, you cannot see any pattern with the p. H or DO/temperature charts. The cleanest was the treated water from the water treatment plant. On the other hand the best habitat for fresh water fish would be the canal next to Lake Hadley because it had the best DO, an okay PH, and a good turbidity. It did have lots of trash, but it also had water plants growing there. All of the locations except the residential waters had trash present. The best p. H readings were the ones in Holly Hill. They were the best because they were closest to neutral, which is what pure water should be. Perhaps this is because of their proximity to the ocean. Question Pond and the Oviedo artesian well had high readings because that water comes from the aquifer and passes through limestone which is a base. Those readings make sense. The really low reading came from the creek at Spring Hammock Preserve. This also makes sense because of the tannin that comes from numerous cypress trees in that area. The chart comparing DO and temperature did not have a consistent connection. The DO was lowest in the residential waters. This makes sense because these waters are compressed to get them through the pipes without using much or any energy. Also these waters do not contain plants and have minerals that reduce the creation of dissolved oxygen. After more research I realized that the time of day samples were collected would also affect the DO, because of the length of time the water would be heated up or cooled. This would need to remain more constant in future studies.

The best turbidity readings were the residential waters and Question Pond, the would have been either naturally filtered or treated at the water plant. One issue I ran into with these tests was the charts provided were not detailed enough to get an accurate reading. Since it was designed for water, the p. H chart should have only ranged from 6 to 9 with more color variants in between so we could have a wider range of possible qualities. Same with the DO. This experiment is more of the first step in screening water quality. More elaborate testing is needed to determine if it is truly safe for animals and people to drink. Additional tests should be done. One key element is the presence of nitrates, which would tell how much fertilizer runoff there was from local agriculture and leaking sewage or septic. High nitrate levels cause fish to die and people to get sick. Acid rain changes p. H. Ammonia can be toxic to aquatic animals but is used by plants for growth. Coliform is another test that should be performed, because it is a good indicator of contamination. Copper is another element at that is toxic to both plants and aquatic animals. Phosphate is a common pollutant in Florida, which causes algae blooms, and so this needs to be tested too. Bacteria is another test that should be done. Further testing of this kind requires much more money and highly specialized equipment. One factor which demonstrates Florida’s surface water contamination was identified by mere observation, and that is human pollution. Human pollution was detected at each measuring site that was not a private residence. This aspect of pollution can quickly destroy ecosystems, yet should be easy to control by following basic human decency.

Conclusion From my observations and experimentation, I learned that the biggest, and most obvious problem facing Central Florida’s natural surface waters is simple human garbage. I was surprised by the amount of trash that I found at each spot, except for the two private residential water sources. Most of the trash was recyclables – cans, plastic and glass bottles, paper, plastic bags, and cardboard. This contributing factor is totally preventable if everyone would make a conscious effort to recycle or throw away their trash. This project helped me to realize that environmental clean-ups do make a significant impact on water quality, for our environment and the wildlife that lives in it.

Bibliography http: //water. epa. gov http: //www. worldwatermonitoringday. org/ http: //islandwood. org/kids/stream_health/Data/DO_Ranges. ht ml http: //www. freedrinkingwater. com/water-education 2/710 oxygen-water. htm http: //water. epa. gov/drink/contaminants/basicinformation/nitr ate. cfm Special thanks to my dad for driving me around for some of my tests and helping me photograph sites. And, to my mom, for assisting me with graphs, photographs, maps, and proofreading.