Tissue Regeneration Tissue regeneration Cells Growth Factors Biomaterials
組織再生 Tissue Regeneration
Tissue regeneration Cells 細胞 Growth Factors 生長因子 Biomaterials 生物材料
血小板 a-顆粒 PDGF VEGF TGF-b 1 FGF EGF
Tissue regeneration Cells 細胞 Growth Factors 生長因子 Biomaterials 生物材料 ?
利用真菌做為生物醫 學材料 Application of fungal materials
Why fungal materials? • Fungi provided wide range of products from food to drugs • Cell wall composition • Polysaccharides and chitin/chitosan (幾丁質、幾丁聚糖)
Fruiting bodies of Ganoderma ?
Residue of extractions 廢渣如何利用? 主要為Cell wall組成
SACCHACHITIN之處理流程 1 N Na. OH H 2 O 2 可製成膜狀
Chemical Analysis 化學分析 • • TLC GC GC/MS NMR
SACCHACHITIN 分析結果顯示主要成分如下 50 -60% Polysaccharide 40 -50% N-acetyl glucosamine
Is SACCHACHITIN safe and functional? 安全性及有效性 Safety Cytotoxicity test, Immunogenecity Animals Rat Guinea pig Canine Inhibition Enzyme and Microbes Human/Clinic trials
Fibroblasts Control SACCHACHITIN 遷移方向 BESCHITIN W 促進遷移 B-Glucan
人類角質細胞也有相同結果 Growth curve of human keratinocyte in the addition 0. 01% SACCHACHITIN suspension.
SACCHACHITIN 6 days Control 6 days Fig. 6. Growth of keratinocyte in suspension of SACCHACHITIN in evaluating of cytotoxicity.
紗 布 紗布 紗布 SACCHACHITIN 7 DAYS 21 DAYS
傷口面積變化 一種日本CHITINT商品 SACCHACHITIN
For pets 為寵物製造之 SACCHACHITIN 10%混合CMC軟膏
人體試驗 Patients • Totally 47 patients were treated with SACCHACHITIN. • All the patients suffered chronic skin ulcer for over 6 months with 3 -10 cm wound in diameter. • All the patients were treated with chemotherapy or other possible treatment, previously. • 36 patients were with satisfactory healing. • The other 11 patients were lost their contact.
66 years old male Infection after open chest operation for 7 months Treatment of SACCHACHITIN 2001/4/16 2001/4/28
Male, 63 yr Old Misuse of steroid drug, Chronic ulcer of left limb for 1 year Daily treatment of SACCHACHITIN 2001/4/10 Evident granulation tissue regenerated 2001/5/7
Pressure sore-1 Male, 79 yr old 2002/6/27 2002/7/11 2002/9/19
Pressure sore-2 Female, 80 yr old 2002/8/15 2002/9/15
What is the mechanism that SACCHACHITIN functioned as a wound healing accelerator? One of the properties of fungal cell wall is to binding lytic enzymes in environment as a defense.
Polysaccharide as matrix Chitin as network Fungal cell wall configuration
? To trap lytic enzymes (MMPs, proteases, lipase… from bacteria)
酵素環境 MMPs (Matrix Metallo-proteinases) MMP-2, MMP-9 the collagenases
Inflammation • Early stage platelets aggregate blood coagulation • Late stage Neutrophils, monocytes Macrophages---bacteria
Matrix metallo-proteases (MMPs) • MMPs were produced by Neutrophils. • MMPs hydrolyzed collagen piling as well as the growth factors. Association of MMPs and chronic ulcer has been reported.
Enzyme in vitro test supported that… 27 k. Da Binding effect of SACCHACHITIN on MMP-9
Quantitative analysis of MMP-9 protein levels in skin wound tissue
- Slides: 49