TimeScale Pan Genome Analysis of Horizontal Gene Transfer

  • Slides: 15
Download presentation
Time-Scale Pan Genome Analysis of Horizontal Gene Transfer in Heterogeneous Bacteria Mixture Jung, Young

Time-Scale Pan Genome Analysis of Horizontal Gene Transfer in Heterogeneous Bacteria Mixture Jung, Young Kwang and Jung, Woo Chul

Index I. Introduction II. Method III. Experiment IV. Discussion

Index I. Introduction II. Method III. Experiment IV. Discussion

Introduction Pan genome Core genome Accessory genome Specific genes Horizontal gene transfer Conjugation Transduction

Introduction Pan genome Core genome Accessory genome Specific genes Horizontal gene transfer Conjugation Transduction Transformation

Introduction Question : How can pan genome be changed by the horizontal gene transfer?

Introduction Question : How can pan genome be changed by the horizontal gene transfer? Hypothetic experiments!!

Method Donor Bacteria X Resistance to antibiotics b HGT Resistance to antibiotics a Recipient

Method Donor Bacteria X Resistance to antibiotics b HGT Resistance to antibiotics a Recipient bacteria A

Method antibiotics a, b 30 min 60 min 90 min

Method antibiotics a, b 30 min 60 min 90 min

Experiment 1 • HGT experiment of segregated sample Bacteria X Bacteria A Bacteria B

Experiment 1 • HGT experiment of segregated sample Bacteria X Bacteria A Bacteria B Bacteria C Bacteria D Bacteria A colonies

Exp. 1 result • Trend in number of colonies with differentiated waiting time in

Exp. 1 result • Trend in number of colonies with differentiated waiting time in HGT experiment of isolated recipient bacteria A. A Time(min) 30 4 60 7 90 11 120 17 Number of colonies Num. of colonies 18 16 14 12 10 8 6 4 2 0 A 30 60 90 120 Time(min)

Experiment 1 • HGT experiment of mixed sample Bacteria X Bacteria A, B, C,

Experiment 1 • HGT experiment of mixed sample Bacteria X Bacteria A, B, C, D Mixed-species colonies

Exp. 1 result • Hypothetical trend in the heterogeneous HGT sample compared with isolated

Exp. 1 result • Hypothetical trend in the heterogeneous HGT sample compared with isolated one. A Time(min) 30 4 3 6 60 7 5 12 90 120 11 17 20 A' in mixed A" in mixed sample 8 14 14 19 A Number of colonies Num. of colonies 15 A' A" 10 5 0 30 60 90 120 Time(min)

Experiment 2 - Sequencing • Sequence each of the samples separately; before HGT, after

Experiment 2 - Sequencing • Sequence each of the samples separately; before HGT, after individual HGT, and after mixed HGT. Bacteria X Isolate transformed A Bacteria A, B, C, D Bacteria A Mixed-species colonies Bacteria X Bacteria A colonies

Experiment 2 - Sequencing • Construct a pan genome as either a group of

Experiment 2 - Sequencing • Construct a pan genome as either a group of species in the mixed sample, or group of samples of same a species in distinct contexts each. Species A Species B Normal A Or Speci es C A wt. Individual HGT A wt. Mixed HGT

Discussion • Limitation 1. Preparing the antibiotics is difficult. 2. Cell division affects on

Discussion • Limitation 1. Preparing the antibiotics is difficult. 2. Cell division affects on experimental result. 3. Low viability of the isolated species in the culture medium.

Discussion • Meaning & Further issue 1. Bacterial community can affect the velocity of

Discussion • Meaning & Further issue 1. Bacterial community can affect the velocity of HGT. 2. Cell to cell genome variation can be affected by HGT. 3. Verify the Specified gene with further experiments.

Q&A THANK YOU

Q&A THANK YOU

Gene Linkage Gene Mapping Human Genome Project GeneGene Linkage Gene Mapping Human Genome Project Gene
New Pan Tilt Introduction PAN TILT Device PanNew Pan Tilt Introduction PAN TILT Device Pan
Virusmediated gene transfer Virusmediated gene transfer q ViralVirusmediated gene transfer Virusmediated gene transfer q Viral
GENE TRANSFER TECHNIQUES INTRODUCTION Gene transfer is toGENE TRANSFER TECHNIQUES INTRODUCTION Gene transfer is to
Gene transfer in plants INTRODUCTION Gene transfer isGene transfer in plants INTRODUCTION Gene transfer is
Gene Transfer in Plants Gene Transfer in PlantsGene Transfer in Plants Gene Transfer in Plants
Human genome organisation Human genome The human genomeHuman genome organisation Human genome The human genome
Genome Structure Kinetics and Components Genome The genomeGenome Structure Kinetics and Components Genome The genome
What is in a genome Genome The genomeWhat is in a genome Genome The genome
Genome Annotation Dr Hilal AY Genome annotation GenomeGenome Annotation Dr Hilal AY Genome annotation Genome
Genome editing 1 Genome editing Genome editing orGenome editing 1 Genome editing Genome editing or
Human genome organisation Human genome The human genomeHuman genome organisation Human genome The human genome
Genome Projects Maps Human Genome Mapping Human GenomeGenome Projects Maps Human Genome Mapping Human Genome
Transferencia horizontal de genes Horizontal transfer contrasts withTransferencia horizontal de genes Horizontal transfer contrasts with