Things to Do Today Observe Unknown gm results
Things to Do Today • Observe Unknown gm (-) results • Start Urinalysis • Start gm (+) ID – – Check for hemolysis Catalase test Coagulase test Direct stain colonies • Observe Throat cultures – Check for hemolysis – Catalase test – Gm stain “isolated colonies”
Oxidase Test Negative Positive
Urease Test Negative Positive
Methyl Red Test Negative Positive
KIA Slants uninoculated
KIA Slants uninoculated
Citrate test Negative positive
The Enterotube Multitest System
The API System: Various species of Enteobacteriaceae are shown here with differences in reactions that enable them to be identified
Things to Do Today • Observe Unknown gm (-) results – Perform oxidase test with culture from KIA slant – Observe Urease tubes – Perform MR test by adding 6 -8 drops MR reagent to the culture – Observe KIA slants for the various reactions
Three species of Candida can be differentiated in mixed culture when grown on CHROMagar Candida plates
Identification of urinary tract pathogens with differential media (CHROMagar)
EMB with E. coli Used with permission
EMB Enterobacter aerogenes Pseudomonas auginosa
Relative Frequencies of Nosocomial Infections I Fig. 15 -22 John Wiley
Relative Frequencies of Nosocomial Infections II Fig. 15 -19 John Wiley
Things to Do Today • Start Urinalysis – Do a 4 quadrant streak with the urine sample using a metal loop onto the EMB plate – Perform the “$” streak with the urine sample using the plastic loop onto the BAP plate
A tale of two Staphs
BAP Hemolytic Reactions Used with permission
Throat Swab Used with permission
Catalase test Positive negative
Coagulase test • Place a drop of the red latix suspension on the card. • Mix a tiny portion of the colony into the mix.
Things to Do Today • Start gm (+) ID 1. 2. 3. 4. • Direct stain with Crystal Violet Check for hemolysis Catalase test Coagulase test Observe Throat cultures (Do in this order) 1. 2. 3. Check for hemolysis Catalase test and find cat (-) colony Gm stain “isolated colonies” on slide with throat swab
BAP Hemolytic Reactions gamma Alpha hemolysis Beta hemolysis
Types of hemolysis: A: Alpha or partial hemolysis of red blood cells results in a greenish zone B: B-hemolysins, which produce complete breakdown of hemoglobin, causing clear zones to form around colonies on blood agar A B
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