Thermo Scientific TSQ 8000 Triple Quadrupole GCMSMS Unstoppable

Thermo Scientific TSQ 8000 Triple Quadrupole GC-MS/MS Unstoppable productivity; Uncompromised MS/MS simplicity

Triple Quadrupole GC-MS/MS is an essential part of a costeffective, high productivity analytical method in today’s laboratory 2

Many laboratories are already investing in and exploring GC -MS/MS as a tool to obtain a competitive edge in their analyses 3

Routine laboratories face tough challenges • High sample loads/short deadlines • Keeping sample analysis costs down with more and more challenging LODs and matrices • Integrating and maintaining new methods and technologies into production workflows to remain competitive 4

What‘s required. . • Realization of the productivity advantages of high performance GC -MS/MS • Minimizing the impact of adoption and implementation to current laboratory operations 5

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Food Safety “To make the productivity advantages of high performance GC-MS/MS easy to achieve and available routinely; especially for high-throughput laboratories. ” Environmental Forensic/Toxicolog y Design philosophy Bioanalysi s 7

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• Routinely achieve regulatory detection limits with confidence • Use highly powerful MS/MS to bring new capability • Not just MS/MS performance • High Sensitivity in MS mode for Full Scan/SIM work 9

• Designed for more robustness; for more uptime and more results reported • Powerful SRM enables optimized sample preparation for faster results • Consolidated, faster methods with more compounds in one run 10

• Fast and simple route to high performance MS/MS with integrated software tools like Auto. SRM • Automatically optimized instrument methods for maximum analysis sensitivty with t-SRM • Synchronized instrument and data processing methods for maintaining efficient workflows 11

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Routinely achieve regulatory detection limits with confidence Dichlorvos in mixed herbal extract at 5 ppb Full Scan SIM SRM 13 • Cut through chemical background • High selectivity in matrix compared with single stage MS • Selected Reaction Monitoring (SRM) • S-shaped curved ion guide lens • High precision at low concentrations • Femtogram level detection • Confirming ions

Highly powerful MS/MS to bring new capability Low-level multi-drug class screen in oral fluid. . Cocaine & BZE at 0. 8 ng/m. L THC and THCA at 0. 2 ng/m. L (all with quantitative and confirmatory ions) • Use the high speed & sensitivity of the TSQ™ 8000 GC-MS/MS • For more compounds • More confirmatory ions • Achieve low noise and clean peak detection • Less manual integration changes • Fast data processing • Add versatility • Easy addition of new compounds to methods 14

Not just MS/MS performance Full Scan SIM Lindane isomers 40 ppb in cucumber in simultaneous full scan/SIM 15 • High full scan spectral quality • Searchable with NIST etc. • High sensitivity SIM & Full Scan • Virtually indistinguishable from a single quadrupole instrument • Dual acquisition modes • Quan & qual in the same run

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Designed for more robustness; for more uptime and more results reported • Design based on strong reputation • Thermo Scientific ISQ Single Quadrupole GC-MS • Innovative GC modularity • Thermo Scientific TRACE 1300 Series GC • Optimized maintenance 17 Before EU limit for LOQ %RSD • Routine-grade robustness • Thermo Scientific Extracta. Brite ion sourceno-vent maintenance • Heated sample path zones for reduced ion burn %RSD pesticides (10 ppb) before and after 1, 400 vegetable matrix injections After EU limit for LOQ

Powerful SRM enables optimized sample preparation for faster results 1, 3 –dichlorophenol, 1, 4 dichlorophenol and 1, 2 dichlorophenol (left to right) in ASE soil extract at 5 ppb. • Explore generic sample approaches • Method consolidation • Eg. Qu. ECh. ERS • Reduce number of steps • Pre-concentration • Clean-up • Bring sample preparation onboard TSQ 8000 • Thermo Scientific Tri. Plus RSH autosampler 18

Consolidated, faster methods with more compounds in one run • Method consolidation • Combine separate MS analyses • Driven by selectivity of SRM • Faster run times • Compress chromatography • Using MS/MS resolution • Clean peak detection • Less manual integration changes • Fast data processing Real example of method consolidation on the TSQ 8000. Five SIM methods combined to form a powerful MRM method for 350 pesticides (700 transitions) 19

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A fast, simple route to high performance MS/MS SRM Optimization Auto. SRM Method Sync Starting Point Inst. Method 21 TSQ 8000 Thermo Scientific Trace. Finder SW Unknown SRM Transitions • The very beginning • or a SIM Method Known SRM Transitions • From an existing method • or SRM Transition list Optimized SRM Transitions • From Compound Data Store • or existing TSQ 8000 method • Integrated SRM management tools • SRM creation and method management independent of your starting point

Fast , simple route to high performance SRM with Auto. SRM 1) Precursor ion selection 2) Product ion selection 3) Collision energy optimization 22 • Auto. SRM manages the SRM method development workflow • From discovery through to final optimization

Automatically optimized methods for high sensitivity with t-SRM • Retention timed-based acquisition • Simple method management • No more window calculations • Timed-SRM (t-SRM) • RT information from user or compound data store (CDS) • Optimizes duty cycle • Ensures maximum method sensitivity, precision and accuracy. • Peaks never on the edge of windows • Timed-SIM (t-SIM) also possible 23

Synchronized methods for maintaining efficient workflows • Method. Sync synchronizes acquisition and processing methods • Exchange SRM and RT information • Instrument method, • Trace. Finder™ SW • CDS 24

Brilliant design, brilliant results Choose between nitrogen or argon for collision gas. No expensive helium is required Full source removal without venting, including ion guide surface. No wire connections are necessary, and never vent to clean your instrument. Curved ion guide enables off-axis optics to effectively eliminates neutral noise Super fast analyzers switch between Full Scan, SIM and MRM in milliseconds. Heated ion guide protects main quadrupole sets so they never have to be cleaned or replaced. Dual Filaments for Prolonged Uptime 25

Unstoppable productivity starts with TRACE™ 1300 Series GC • Instant Connect (i. C) injector and detector modules • User switching in minutes • For maintenance or flexibility • Low thermal mass design throughout • Fast heating and cooling for higher sample throughput • Tri. Plus™ RSH autosampler • Automation of sample preparation steps • Dilutions • Addition of standards 26

Trace. Finder; maximum productivity workflow-oriented software • Workflow-centric • Batch acquisition • Integration to TSQ 8000 method • Data processing • User Role Capabilities • Simplifies routine workflow • Active Reporting • Dynamic updates based on real-time changes • Application Variants • Environment & Food Safety • Clinical Research & Toxicology 27

TSQ 8000: Core values for the routine choice in GC-MS/MS High performance 1 Unstoppable productivity MS/MS simplicity 28 3 2

Your next competitive advantage 29