The Microscope The Microscope Enables you to see

The Microscope

The Microscope • Enables you to see things that are too small to be seen with the unaided eye. – Allows you to look more closely at the finer details of things.

What you see Blood What the microscope sees

What you see Fly What the microscope sees

What you see Leaf What the microscope sees

What you see Eyelash What the microscope sees

What you see Strawberry What the microscope sees

Microscope Parts Eyepiece (10 X) Coarse Adjustment Fine Adjustment Arm Body Tube Nosepiece Scanning Objective (4 X) High Power Objective (40 X) Base Stage Clips Diaphragm Light Source

Parts at Work • Eyepiece – What you look through. • by itself enlarges the specimen by 10 X • Objectives – Scanning Power (4 X) = to locate and focus the specimen, allows you to see the largest amount of a specimen. (Red) – Low Power (10 X) = to examine details (Yellow) – High Power (40 X) = to examine the finest details (Blue)

Parts at Work • Coarse Adjustment – to focus in the specimen under scanning and low power. • Fine Adjustment – to focus in the specimen under high power ONLY. • Arm & Base – used to carry the microscope. • Nosepiece – holds the objective lenses.

Parts at Work • Stage & Stage Clips – holds the slide with specimen securely. • Diaphragm – adjusts the amount of light shining through specimen. • Light Source – provides light that shines through the specimen.

Magnifications Part Eyepiece Scanning Objective Low Power Objective High Power Objective Magnification 10 X 4 X 10 X 40 X

Total Magnification • The total amount a specimen is magnified due to the combined magnifications of the eyepiece and objective lenses being used. Eyepiece X Objective = Total Magnification 10 X 5 X 50 X 15 X 225 X 10 X 100 X 20 X 40 X 800 X

Total Magnifications Eyepiece Objective Total Magnification 10 4 40 10 10 10 40 400 1. Which total magnification would allow you to see: a) The largest amount of the total specimen? 40 X b) The finest details of the specimen? 400 X 2. Which set of lenses would you use to find and focus a specimen? 40 X

Total Magnification B. 1. Scanning Power (40 X) _____ A. – Used to find a specimen A. 2. Low Power (100 X) _____ – To view more detail of the specimen. B. C. 3. High Power (400 X) _____ – To view the greatest detail of the specimen. C.

Field of View • The diameter of the circle of light that you see when looking through the eyepiece is called the Field of View. • What is the field of view as seen in the image? 15 mm _______ 1. 5 cm or _______

Field of View • As the power of the microscope becomes greater the field of view gets smaller. – When measuring field of view always read it from the diameter. Scanning Power: (40 X) 4. 0 mm High Power: (400 X). 5 mm

Field of View • If every = 1 mm, what would be the diameter of this field of view? A. 6 mm • If every = 1 mm, what would be the approximate size of letter “e”? 2 mm B.