The Microscope 1 Types of microscopes The compound

The Microscope 1

Types of microscopes �The compound light microscope �The Dissecting microscope �The electron microscope 2

�The microscope used most commonly in cell study �For study with this microscope, the specimen must be thin enough for light to pass through it easily 3

Compound Light microscope �It has 2 lenses �Light passes through a specimen, then passes through the objective lens �The enlarged image is magnified AGAIN by the eyepiece lens 4

�The dissecting microscope allows you to see large specimens in threedimensional quality �The electron microscope produces magnification of more than 100, 000 times 5

Parts and functions �The tube that connects the oculars(the eyepiece) to the revolving nose piece which holds objectives �Body tube 6

�the lens on the top of the microscope that you look through �Eyepiece (ocular lens) 7

�Low-power objective: �The shorter objective{yellow} �Contains lenses that magnify objects 10 times 8

�the low power lens is more zoomed out and you can see a larger area so you see more of your image 9

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�High- power objective: �The longer objective{blue} �Contains lenses that usually magnify 40 times 11

�When viewing under the high power objective {the blue one}, you can see better details of the image, but you cannot see as much of the image 12

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Coarse adjustment �the larger knob on the microscope �When you move this knob, it moves the stage up or down 14

Fine adjustment �This is the small knob on the microscope �The fine adjustment sharpens the image {makes you see clearer} under low power 15

�Stage: �platform for holding the slides �Stage clips: �holds the slide on the stage 16

�used for carrying the microscope �Arm and Base 17

Nosepiece �This rotates the objectives 18

�this controls the amount of light passing through a specimen �The diaphragm 19

Ø The circle of light you see when you look through the microscope ØField of view 20

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Microscope “RULES” �Never use the coarse adjustment under high power because you can break the slide 22

What is the formula for total magnification? �To determine the total magnification, you multiply the eyepiece by the objective lens �Total magnification refers to the total amount that the image is enlarged 23

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�Under the low power objective, the field of view gets larger �Under the high power objective, the field of view gets smaller 26

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�How does an image appear under the microscope? �The final image appears enlarged, upside down and backward 29