The Kinetics of a Palladium Precursor Tobacco Mosaic

The Kinetics of a Palladium Precursor – Tobacco Mosaic Virus Reaction Adi Banerjee 1*, Mayo Adigun 1, Dr Michael T. Harris 1 School of Chemical Engineering, Purdue University , West Lafayette, IN Abstract Methodology This study of the kinetics of a nanomaterial synthesis reaction specifically focuses on the reaction between a palladium precursor and the Tobacco Mosaic Virus where Pd ion concentration is measured over time using UV-Visible, Atomic Absorption and X-Ray Absorption Spectroscopy methods. Conclusion and Future Work The findings using UV Visible Spectrometry do not exactly correlate with those from X Ray Absorption at ANL, due to the numerous sources of experimental error which correspond to significant fluctuations in results. TMV 1 Cys This study would elucidate the understanding of the effectiveness of different spectroscopy methods to measure concentration and thus obtain more information about the general kinetics of reactions between metals and biotemplates. ve e mo um Re vol 2 O all d H sm d +A Na 2 Pd. Cl 4 Precursor 55°C, 12 hr n i e t t r e e v et u tr c rom e ct s In spe The biggest concern is that the presence of external particles may influence all absorbance measurements. Atomic Absorption proved to be a challenge as the Hollow Cathode Lamp specifically for Pd was faulty. However there have been many qualitative findings throughout the summer which will open up new avenues for further research using other spectroscopy methods. + h t e a g B ifu e Ic ntr Ce Remove liquid + filter + add to Cuvette Figure 1: Structure of Tobacco Mosaic Virus C Michael Hogan (2010). Virus. Encyclopaedia of Earth. Quantiative Findings Introduction The reaction is between a Pd precursor (Na 2 Pd. Cl 4) and genetically modified TMV (TMV 1 -cys). Pd supernatant concentration is measured over time. This is a “special” redox reaction in that there is no external reducing agent i. e. a functional group on the coat protein of the virus reduces the Pd ions to particles. The purpose of this experiment is to provide confirmatory data and analysis to X Ray Absorption Spectroscopy done at the Argonne National Laboratory, using both Atomic Absorption and UV-Visible Spectroscopy methods. Date of Creation Equation Correlation Coefficient 13/6/14 Abs=6. 321×Conc+0. 00777 0. 995 24/6/14 Abs=7. 624×Conc-0. 0120 0. 984 8/7/14 Abs=9. 7864×Conc+0. 00275 0. 984 Figure 4: Alfalfa Mosaic Virus (AMV) Figure 5: Barley Stripes Mosaic Virus (BSMV) under a Coloured under an SEM TEM Antoni Luque (2010). A physical model describes the structures of viral Anon. (Unknown) BSMV, TEM. Science Photo capsids. Universitat de Barcelona References Jung-Sun Lim, Seung-Min Kim, Sang-Yup Lee, Eric A. Stach, James N. Culver, Michael T. Harris. (2010). Biotemplated Aqueous-Phase Palladium Crystallisation in the Absence of External Reducing Agents. Nano-Letters. 10 (1), 3863 -3867. Table 1: Small Concentration Calibration Equations on Different Dates Average Supernatant Concentration vs. Time Amy K. Manocchi, Soenke Seifert, Byeongdu Lee, Hyunmin Yi. (2011). In Situ Small Angle X-ray Scattering Analysis of Palladium Nanoparticle Growth on Tobacco Mosaic Virus Nanotemplates. Langmuir. 27 (1), 7052 -7058. Figure 2: Schematic diagram of a Atomic Absorption Spectrophotometer Brian Tissue (1996). Atomic-Absorption Spectroscopy. Science Hypermedia Concentration (mg/ml) 0, 012 0, 01 Acknowledgements 0, 008 0, 006 I would like to thank the SURF Program for allowing me this opportunity to experience research on a first hand basis. 0, 004 I would also like to thank Dr Michael T. Harris for allowing me to use all the facilities, and his graduate student group who have assisted me in my endeavours. 0, 002 Figure 3: Schematic diagram of a UV-Visible Spectrophotometer Brian Tissue (1996). UV-VIs Spectroscopy. Science Hypermedia 0 0 100 200 300 400 Time (minutes) 500 600 Graph 1: Plot of Supernatant Concentration over Time 700 800 Lastly, all the work isn’t possible without the underlying effort of my graduate mentor Mayo Adigun. I thank him for the tremendous effort throughout the summer.