The Halogenation of an Organic Compound CHEM 113

  • Slides: 1
Download presentation
The Halogenation of an Organic Compound CHEM 113 Section 001 Professor Pham Mikaela Brutico,

The Halogenation of an Organic Compound CHEM 113 Section 001 Professor Pham Mikaela Brutico, Thomas Niuman, Jeremy Dippel Abstract Results In this experiment we studied light absorption of a reaction consisting of Curcumin and a chlorinating agent, NChlorosuccinimide (NCS) The reaction mixture was dissolved in Ethyl Acetate and Hexane. UV-VIS Spectroscopy was then used to analyze the absorption rate over time as the reaction progressed. Using spectrum analysis we were able to determine that the chlorination of our curcumin sample was completed after 24 hours. Curcumin powder with Tumeric roots behind Method We sampled 10 m. L of Ethyl Acetate Hexane, then weighed 50 mg of Curcumin and determined the amount of NCS needed from the amount of Curcumin weighed out (1. 2 equivalence) and placed them in a vial. Curcumin mixture containing NCS dissolved in Ethyl Acetate Hexane Introduction Curcumin is the principal curcuminoid of turmeric, a plant in the ginger family. It is a traditional spice in Indian and South Asian food. It has been studied for its health benefits, and its possible medicinal uses include treating certain forms of cancer, arthritis, and Alzheimer’s disease. Initial absorption line Next, we dissolved our curcumin into our solution in the vial. The vial was allowed to mix using a magnetic stirrer. The time from the starting point to the end point was recorded using a spectrophotometer. Conclusion Ethyl Acetate Hexane was used as blank (200 -800 wavelength- UV and visible) to use UV-VIS spectroscopy Finally, we tested our solution. We produced a sample of one drop of solution with Ethyl Acetate Hexane and mixed well. We repeated this multiple times to test the wavelength. Initial and final absorption lines In this experiment we aimed to follow the course of a reaction, specifically the chlorination of curcumin. After 24 hours we observed a new absorbance at Curcumin is useful in the medical world, but it has a low absorption rate, metabolizes quickly, and is insoluble in water. It must be dissolved in a solvent to allow for any concentration. TLC showing a new spot above curcumin after 24 hours ~450 nm in our UV-VIS sectrum indicating the formation of a new compound. The reaction mixture was also analyzed using thin layer chromatography (TLC). TLC also showed the presence of a new spot or a new compound. Further analyses done prior to our experiment confirmed that the new product is the chlorinated form of curcumin. References http: //www. scilogs. com/from_the_lab_bench/curcumin-and-the-big-c/ http: //www. webindia 123. com/spices/images/turmeric. jpg The halogenation process introduces a chlorine atom to curcumin.