The fraction of horizontally transferred genes in Methanosarcina spp. decreases with time well, in some sense date of HGT: Methnosarcinal es, Methanosarcina ceae, Methanosarcina,
Bacterial species • • Morphology of cells and colonies Catabolism (metabolic abilities) Anabolism (auxotrophy) Cell wall components • ≥ 70% by DNA-DNA hybridization, ≤ 5ºC ∆Tm (Wayne et al. , 1987) – 'gold standard'. • ≥ 97% 16 S r. RNA sequence similarity (Stackebrandt and Goebel, 1994)
Gene complements (gene-ome) • text
Pan-genomes • Total set of genes in all strains of a species / genus (Tettelin et al, , 2005): – core ((universal genome): present in (almost) all strains – periphery: present in some strains – unique: (almost) strain-specific
Escherichia + Shigella. Gene occurrence in strains
Sum of three exponents (Wolf&Koonin) y = exp (– 0. 53 x + 8. 30) + exp (– 0. 02 x + 4. 41) + exp (0. 60 x – 12. 27)
Sum of two power laws y = 4400·x– 1. 72 + 674· (32–x)– 1. 26
Add Salmonella – get internal peaks Universal genome of Salmonella Universal genome of Escherichia