THE EFFECTS OF SOLUTION TEMPERATURE ON CATALASE REACTION

THE EFFECTS OF SOLUTION TEMPERATURE ON CATALASE REACTION RATES Mariah Hamilton, Gracie Hobbs, Rose Millay, Annie Miller

PICOT ◦ During catalyzation, does increased temperature or room temperature raise the rate of reaction during the reaction over 200 seconds (0°C, 21°C, 50°C, 55°C)?

Conduction of Experiment ◦ Place 6 m. L of H 2 O in each flask and test tube. ◦ Add 6 m. L of 3% H 2 O 2 in each flask and test tube. ◦ Place one tube in a 0°C ice bath, both flasks on a 50°C hot plate, and leave one tube at room temperature (21°C). ◦ Place 2 drops of catalase into room temperature test tube, place stopper in test tube, and measure reaction on Logger. Pro for 200 seconds. ◦ Once the other test tube and flasks have reached desired temperatures, repeat the process used on the room temperature tube for each. ◦ For both of the heated flasks, place the stirrers in the solutions and turn on the aggravators. ◦ Record the slope of each line.

Analysis of Results ◦ The 0°C solution had a slope of 0. 05834 k. Pa/s. ◦ The 21°C solution had a slope of 0. 03753 k. Pa/s. ◦ The 50°C solution had a slope of 0. 05503 k. Pa/s. ◦ The 55°C solution had a slope of 0. 02032 k. Pa/s. ◦ We concluded that the solution cooled to 0°C had the steepest slope, beating 21°C, 50°C, and 55°C, which makes it most effective at maximizing catalase reactions.