The Effects of Antibiotics on Gastrointestinal Motility and
The Effects of Antibiotics on Gastrointestinal Motility and Gut Microbiota Catherine Chen Illinois Mathematics and Science Academy April 28, 2016
Possible Perturbations of Gastrointestinal Tract Host Physiology Microbial Metabolites/ Metabolic Activity Serotonin Pathway Bacterial Community SCFA Production
Hypothesis Antibiotic use affects GI motility through alterations in gut microbial community and/or changes in the host physiology. Gut microbiota GI motility
Goals 1. Determine effects of antibiotics on intestinal transit time and host physiology. 2. Examine antibiotic effects on microbial structure and metabolic profile.
Antibiotic usage Antibiotic Target Treats Cefoperazone gram + gram - infections: peritonitis Vancomycin gram + diarrhea causing bacteria Neomycin gram- infections, used in ointments
Study Design -Abx in drinking water +Cef in drinking water +Vanco in drinking water +Neo in drinking water Measured Transit time Collected luminal contents 16 s SCFA Tissues m. RNA (q. RT-PCR)
Gastrointestinal Transit Time
Bacterial Community Changes in Antibiotic. Treated Luminal Contents
Short Chain Fatty Acid Changes in Antibiotic. Treated Luminal Contents Propionate Butyrate Acetate Grider and Piland. AJP. 2007.
Short Chain Fatty Acid Changes in Antibiotic. Treated Luminal Contents
Antibiotic Treatment Effects on Gene Expression Levels
Antibiotic Treatment Effects on Gene Expression Levels
Antibiotic Treatment Effects on Gene Expression Levels
Antibiotic Treatment Effects on Gene Expression Levels
Conclusions Antibiotic treatment can significantly: • increase GI transit time • alter intestinal microbial community. • decrease SCFA production • affect serotonin pathway
Summary Microbial metabolite production Bacterial community structure GI motility Gut microbiota Delayed Transit Time Constipation
Acknowledgements • Dr. Chang, Dr. Touw, & the rest of the Chang lab at University of Chicago • All the staff of the Student Inquiry Research Program at the Illinois Mathematics and Science Academy
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