the BIG role of SMALL BIOMOLECULES Anna R
the BIG role of SMALL BIOMOLECULES Anna R. Bramucci Ph. D. Candidate University of Alberta Canada
NASA: Bering Sea April 1998
To what degree do small bioactive molecules control bacterial-algal symbioses? growth promoters olfactory signal bad taste molecules antibiotics
Jekyll-and-Hyde Chemistry of Phaeobacter gallaeciensis Seyedsayamdost*, Case*, et al. (2011) Nat. Chem.
Early Mutually Beneficial Symbiosis: BS 107 Releases TDA in Simulated Healthy and Dying Algal Cultures antibiotics (TDA) p. CA
lignin breakdown Late-Stage Switch to Pathogenesis: p. CA Roseobacticide —p. CA TDA + p. CA TDA —p. CA Roseobacticide + p. CA Roseobacticide BS 107 responds to p. CA and releases Roseobacticides in Simulated Healthy Algal Cultures
Switching Chemistry Early Stage: Symbiosis Late Stage: Pathogenesis lignin breakdown p. CA TDA growth promoters Roseobacticide
2 N Coccolithophore CCMP 3266 Algal Cell Counts Bacterial Plate Counts
2 N Coccolithophore CCMP 3266 PAM Minimal Fluorescence (F 0) Indicator of Algal Health Yield [(Fm-F 0)/Fm] Indicator of Health of Photosystem II
Future Ph. D. Project Directions • Identify novel Roseobacticides • Test previously identified small bioactives on selected Emiliania huxleyi CCMP strains • Identify novel small bioactives that increase algal health or stimulate bloom collapse Seyedsayamdost*, Case*, et al. (2011) Nat. Chem.
Arctic Ocean Field Season • Expose natural communities to bioactive small molecules • Identify how this impacts the composition, abundance, and health of the natural populations bloom ocean waters No shift Loss of Natural Community DGGE
Acknowledgements.
QUESTIONS? ?
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