The basics of fluorescent probe Academic skills in

The basics of fluorescent probe Academic skills in forest sciences Name: Changling xu UEF// University of Eastern Finland Date: 27/9/2020

optical phenomenon “cold light, ” similarly phosphorescence fixed energy for given fluorophore https: //www. wisegeek. com/ biochemical and molecular research what is fluorescence? fluorescent probe? Signalling subunit, spacer, binding subunit, analyte, output signal. 17. 12. 202 2 Author of the presentation, Name of the event 1

How they work

PET (photo-induced electron transfer) ICT (intramolecular charge transfer) FRET (fluorescence resonance energy transfer) Excimer / exciplex fluorophore, sensitivity spacer, linker, receptor, selectivity, specificity UEF// University of Eastern Finland groups

Method spectrophotometry (Fluorescence) electrochemical analysis atomic absorption Disadvantage Advantage Easy, low cost, Interference (co-ions) sensitivity, reproductivity fast, low cytotoxicity Cell damage, high consumption sensitivity, reproductivity High consumption, Good specificity, high low LOD, accurcy easy, fast equipment damage spectrometry Cell damage nductively couple Cell damage Excellent specificity, plasma high cost, reproductivity UEF// University of Eastern Finland

High fluorescence selectivity, High affinity ( LOD 8. 36 × 10− 9 M, (ka) 5. 14 x 104 M− 1 Bioimaging application Image 7. Fluorescence microscope images of the He. La cells. After incubation with the TSA (10 µM) for 60 min (a); Bright -field images (b); after incubation with the TSA (10 µM) for 30 min and then further incubated with Al 3+ (10 μM) for 30 min (c) and 60 min (d). Fast response Broad range of p. H 17. 12. 202 6 Author of the presentation, Name of the event 1

Fluorescence Fluorescent probe Photoluminescence (PL) Tool, source： https: //cn. bing. com/images/ Recognition 17. 12. 202 1