Thalassemia screening test Pichanee Chaweekulrat MD Lab Thalassemia

Thalassemia screening test Pichanee Chaweekulrat MD.

Lab ������� Thalassemia screening test 1. subject 1 �� /���� 2. unknown 2 ���� /���� (unknown 1, unknown 2)

Lab ������� Thalassemia screening test 1. DCIP precipitation test 2. One tube osmotic fragility test (OF Test)

Blood sample preparation Subject 1 �� /���� 1. ������������� lancing device ����� capillary tube lancet lancing device อปกรณทใช - lancing device และ lancet - Capillary tube (hematocrit tube) 2 หลอด - Eppendorf tube - ถงมอ - สำลแหง - 70% alcohol - ททงขยะ

Blood sample preparation 2. ��������������� (eppendorf tube)

Blood sample preparation

Thalassemia screening test 1. DCIP precipitation test : Kit reagent ������ KKU-DCIP-Clear Reagent Kit - KKU-DCIP reagent 2 ml - Clearing solution

Thalassemia screening test 1. DCIP precipitation test : ������ 1. ��� automatic pipette ���� 20 μl ����� KKU-DCIP reagent 2 ml 2. ��������� 2 -3 ������� 3. ����� water bath ��� 37°C ��� 15 ���� 4. ���� clearing solution 20 μl ������ 3 ���������

Thalassemia screening test 2. DCIP precipitation test : �������� ����������

Thalassemia screening test 2. One tube osmotic fragility test (OF Test) : ����� Positive • • • ����� thalassemia ��� homozygous Hb E ���� 100% β-thalassemia trait ���� 90% α-thalassemia 1 trait ���� 93% ��������������� ������������� False Positive ���������� 5%

Thalassemia screening test 2. One tube osmotic fragility test (OF Test) : ����� 1. ��� automatic pipette ���� 20 μl ����� KKU-OF reagent 2 ml 2. ��������� 2 -3 ������� 3. ������������� 30 ����������

Thalassemia screening test 2. One tube osmotic fragility test (OF Test) : �������� ��������������� ���������

Confirmatory test 1. Hemoglobin typing 2. Iron studies : serum ferritin, serum iron, total iron binding capacity (TIBC)