Th 1 Biased Progressive Autoimmunity in Aged Aire

Th 1 Biased Progressive Autoimmunity in Aged Aire- Deficient Mice Accelerated Thymic Epithelial Cell Senescence Zhang Jie, Wang Yuqing, Aili Abudureyimujiang, Sun Xiuyuan, Pang Xuewen, Ge Qing, Zhang Yu, Jin Rong Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Beijing 100191, China Figure 7. Th 1 biased condition accelerates senescence of thymic epithelial cells ( A ) Mice were immunized subcutaneously with 5&#x 003 BC; g of OVA in saline or CFA for a total 6 times at 6 -, 8 -, 10 -, 17 -, 19 - and 21 -weekold analyzed at 16 -, 27 - and 39 -week-old. ( B ) Mouse body weight of immunized mice at indicated time points. ( C ) IFN-&#x 003 B 3; - and IL-4 -producing CD 4 + cells in the spleen of immunized mice at indicated time Aging and Disease, 2019, 10(3), 497 -509. DOI: 10. 14336/AD. 2018. 0608 points were detected by intracellular staining. Data are showed as the ratio of IFN-&#x 003 B 3; + &#x 00025; : IL-4 + &#x 00025; . ( D-H ) The numbers of total thymocytes ( D ), DN ( E ), DP ( F ), CD 4 SP ( G ) and CD 8 SP ( H ) thymocytes in the thymus are shown. ( I, J ) The percentage of GFP +hi and GFP -lo cells in the lymph nodes ( I ) and spleen ( J ) after OVA immunization. Each dot represents an individual mouse. ( K ) Ep. Cam + Ly 51 lo m. TEC and Ep. Cam + Ly 51 hi c. TEC in the thymus of two immunized groups were detected by Ep. Cam and Ly 51 staining in CD 45 - gate. Representative density plots ( left ) and ratio of m. TECs versus c. TECs ( right ) are shown. ( L ) The expression of MHCII in m. TEC from two immunized groups. Representative density plots ( left ) and the proportion