Template of Poster for International Seminar AISTSSE October

Template of Poster for International Seminar AISTSSE, October 17 -18, 2019 Ahmad Shafwan Pulungan , D. Sofian Wildan , Siti Nur Inayah , Sutarya Enus , 1, 2 1 1 3 Iman P. Maksum 1, Khomaini Hasan 4, Toto Subroto 1*, and Soetijoso Soemitro 1 1 Department of Biology, Universitas Negeri Medan, Indonesia, 2 Department of Chemistry Education, Universitas Negeri Jakarta, Indonesia, 3. . . etc. *Corresponding authors: shafwan@unimed. ac. id Abstract 2. Solubilisation of rh. PT 2 in different strategies This template was designed to produce a 594 mm x 841 mm (A 1 size) poster in portrait or landscape orientation. We’ve put in the headings we usually see in these posters, you can copy and paste and change to your hearts content! We suggest you use keep black text against a light background so that it is easy to read. Background color can be changed in format-background-drop down menu. Table 1. rh. PT 2 expression at different chaperone modulation. E. coli Size of poster is Because of important function in clot formation thrombin has been utilised in a number of product intended for haemostasis and/or as tissue sealants. The recombinant human prethrombin-2 (rh. PT 2), as a thrombin precursor, is a protease-like protein which is expressed by E. rh. PT 2 (thiol) rh. PT 2 (p. H) S I - ER 2566 Background Chaperone modulation Dna. K, Dna. J, Grp. E, Gro. EL and Gro. ES ER 2566 Dna. K, Dna. J, and Grp. E ER 2566 Gro. EL and Gro. ES Arctic Express Cpn 10 and Cpn 60 S : soluble; I: insoluble 3. Mechanism of chaperone Method A B p. G-KJE 8 Figure 2. Chaperone cycle of (A) Gro. EL and Gro. ES, and (B) Dna. K, Dna. J, and Grp. E. Arctic. Express and ER 2566 -chaperone co-expression successfully expressed soluble rh. PT 2. Gro. EL/Gro. ES and Cpn 60/Cpn 10 are the best chaperone systems for expressing soluble rh. PT 2. Arctic. Express ER 2566 • • ER 2566 Conclusions Expression SDS-PAGE Results The solubilisation of the rh. PT 2 fusion intein-tag was achieved by chaperone modulation. These results revealed that chaperones are tools for protein folding and assists the solubilisation process of rh. PT 2 in E. coli. Results 1. Cloning of rh. PT 2 into p. TWIN 1 A B 6000 1000 M 1 2 p. TWIN 1 rh. PT 2 (thiol) 6000 1000 M 1 2 p. TWIN 1 Acknowledgments This work is supported by grant Penelitian Unggulan Strategis Nasional (PUSNAS) from Directorate of Higher Education, Ministry of National Education, Indonesia. rh. PT 2 (p. H) References Figure 1. Cloning of (A) rh. PT 2 (thiol) and (B) rh. PT 2 (p. H) into p. TWIN 1. M is marker, 1. undigested plasmid and 2. doubledigested plasmid resulting p. TWIN 1 and rh. PT 2. • Soejima et al. 2001. J Biochem. 130, 269 -277 • Strocchi et al. 2006. Proteomics. 6, 193 -206 • Walter and Buchner. 2002. Angew. Chem. Int. Ed. 41, 1098 -1113