Technologies for COVID 19 PROF RINTI BANERJEE FASC

Technologies for COVID 19 PROF. RINTI BANERJEE FASC, FBAO MADHURI SINHA CHAIR PROFESSOR DEPT OF BIOSCIENCES & BIOENGINEERING INDIAN INSTITUTE OF TECHNOLOGY BOMBAY CONFIDENTIAL, IIT BOMBAY

Antiviral Nutraceutical for Prophylactics • Objectives • Develop antiviral formulations based on spices and edible emulsifiers • Mechanism of action: • Direct breakdown of viral envelope due to surface active nature • PICOVRID_N • • • Antiviral nutraceutical drinks High absorption, Stable formulations, taste masked FDA approved, In accordance with Food Codex limits FSSAI approved constituents according to Schedule VI In vitro effectiveness against several viruses including coronavirus Indian Patent filed Completely inactivates clinical coronavirus swab samples in vitro in one hour Strong antiviral and RNAse activity Safe at 2000 mg/kg Confidential, IIT Bombay

Swab Sample 1 after incubation with Cycle threshold on RT PCR formulation for 1 hour, RTPCR N Gene: 18 POSITIVE N Gene: NEGATIVE O Gene: 17 POSITIVE O Gene: NEGATIVE S Gene: 17 POSITIVE S Gene: NEGATIVE MS 2 : PASS MS 2 : NIL Swab Sample 2 Swab sample 2 after incubation with Cycle threshold on RT PCR formulation for 1 hour, RT PCR N Gene: 22 POSITIVE N Gene: NEGATIVE O Gene: 22 POSITIVE O Gene: NEGATIVE S Gene: 22 POSITIVE S Gene: NEGATIVE MS 2 : PASS MS 2 : NIL Swab Sample 3 after incubation with Cycle threshold on RT PCR formulation for 1 hour, RT PCR N Gene: 21 POSITIVE N Gene: NEGATIVE O Gene: 20 POSITIVE O Gene: NEGATIVE PICOVRID_P Herbal Antiviral Therapy for Mild & Moderate COVID 19 • • Planned Three Arm Randomised Controlled Trial Mild to Moderate COVID 19 Standard care plus nutraceutical once a day Standard care plus herbal therapy thrice a day RT PCR of cycle thresholds Clinical recovery Complications Biomarkers Confidential, IIT Bombay

NANOSURF: Aerosols for Pneumonitis and ARDS Complications of COVID 19 • Address mortality associated with ARDS complications of COVID 19 • Unmet medical need, No known treatment • Objectives • Develop aerosol formulations that address the cytokine storm of ARDS (MDI and nebulized, oxygen microbubbles) • Mechanism of action • Acts on alveolo-capillary membrane, Reduces cytokine storm • Pulmonary surfactant mimetic • US 9345716 and Indian Patents Granted • Formulations optimized and extensively validated at laboratory level • Validated against LPS induced ARDS models in rats • Significantly reduces cytokine storm, bronchoalveolar IL 6 levels, bronchoalveolar protein levels, bronchoalveolar oxidant levels • All ingredients GRAS approved • GLP Acute inhalation toxicity done in rats and mice, Safe in accordance with OECD 463 • Trial as Investigational New Drug (Emergency use) Confidential, IIT Bombay

DURAPROT: Wash resistant Antibacterial & Antiviral Coatings for textiles, masks, PPEs • Enhance functionality of regular 3 ply masks, textiles, PPEs • Objectives • Develop wash resistant coatings on textiles with antibacterial and antiviral properties • Mechanism of action • Easily cross linked to textile, Acts on envelope of viruses and cell walls of bacteria Indian Patent filed Strong antiviral effects Resistant to 20 wash cycles Completely inactivates coronavirus samples in clinical swabs in one hour • Scaled up & Licensed to several industry partners • • • Meemansa, Ants Innovations, Ecostyle. Crafts • High breathability, self disinfecting, reusable • Available for licensing and deployment Confidential, IIT Bombay

Strengths and Co-operation • Drug delivery • Encapsulation strategies Interested in • In vivo coronavirus models • Clinical trials in at risk population • Enhance bioavailability • Biodegradable nanoparticle platforms • Nutraceutical • Over The Counter/Herbal • Investigational New Drug • Stabilise actives, controlled release • Pulmonary surfactant mimetic • Ultrasound responsive drug loaded microbubbles for oxygen therapy • Biomaterial strategies for antiviral coatings • Deployment of technologies • Textiles, Masks, PPE coatings rinti@iitb. ac. in Confidential, IIT Bombay