Tanmia Biosecurity Plan Preventing Avian Influenza Virus Infection

Tanmia Bio-security Plan Preventing Avian Influenza Virus Infection in Breeder & Broiler Farms, Hatchery & Feed Plant

Plan Structure l Concepts: - Definition. - Transmission concept. - Clinical signs. - Awareness & notification. - Means of transmission. - Common carries. l Prevention / control program: A- Bio – exclusion. B- Bio – security measures. - Farm specific. - Hatchery specific. - Feed plant specific. l Detailed procedure/work instructions/workshop with procedure farm & vet. managers to be expanded.

CONCEPTS Definitions l Farm l Site : : Comprising several breeder fenced sites within a common fenced boundary connected by a network of roads or a rented farm. Comprising several sheds within a fenced area of the farm. l Hatchery : Isolated and specific for each type of breede or grandparents. l Feed Plant: One specific for grand parents. One common for breeders.

CONCEPTS - (cont. ) Avian Influenza Transmission Migratory birds + Mechanical & Biological Carriers Poultry Nomadic : - Water fowl - Wild birds

CONCEPTS - (cont. ) Clinical Signs / Lesions of AI ( GP & Breeders ) l Severe flock depression. l Decreased feed intake. l Respiratory disease with excessive mortality. l Swollen heads, bluish combs and wattles. l Nervous signs. l Watery droppings.

CONCEPTS - (cont. ) AI Disease Awareness and Notification All poultry staff and workers to immediately report any unusual levels of death or sickness to Wadi production management and veterinarians.

CONCEPTS - (cont. ) AI Virus Main Transmission l AI virus occurs in the droppings and other body discharges (eye , nose, …)of migratory, wild birds, water fowls and infected poultry. 0 l Feaces, discharges and other mechanical means contaminate various items that enter farms and sheds. l Procedures must stress absolute prevention of carrying infected feaces, discharges, diseased and dead birds between farms and into sheds.

Concepts - (cont. ) Common Carriers l Vehicles onto farm, hatchery or feed plant. l People onto farm or hatchery. l Contaminated bedding material. l Equipment entering sheds or hatchery. l Spiking. l Movement of culled and dead birds. l Rodents and insects.

CONCEPTS - ( cont. ) Common Carriers – ( cont. ) l Domestic animals (dogs , cats, …). l Grazing animals (sheep, goats , cows, …). l Wild and domestic birds. l Water sources ( drinking, cooling , showering, . . . ). l Spent hens. l Aerosols (dust ) infection. l Farm shed clean out /disifection.

PREVENTION / CONTROL PROGRAM Bio – exclusion is a principle and discipline l Training and educating staff at all and different levels to improve knowledge and insure procedures follow up. l Farm staff weekly meeting with farm/ site managers to get site managers feed back. l Monthly farm managers meeting with top management to optimize the procedures.

PREVENTION / CONTROL PROGRAM Bio – exclusion is a principle and discipline ( cont. ) l Insure well arranged daily cleaning of farms staff housing and clothes, through the sites assigned committee. l Showering facility, fumigation cabinet and UV desinfection box at each site to be properly used is a must. l Availability of 2 -3 farm coverall and underwear sets to be cleaned regularly person.

PREVENTION / CONTROL PROGRAM Bio – exclusion is a principle and discipline ( cont. ) l General concepts of personal hygiene should be followed. l Supplying farms / sites staff with weekly/monthly food materials and encourage food preparation within the farm/site. l Ensure availability of well equipped, well staffed vet. diagnostic lab.

PREVENTION / CONTROL PROGRAM Bio – exclusion is a principle and discipline ( cont. ) l Employee blood testing to insure negative salmonella status. l Only clean ( avoid misshapen , dirty , floor and hair cracks) hatching eggs to be collected / set. l Ensure formalin treated nest bedding, egg belt cleanout ( brush, blowers ) and continuous H. E collection.

PREVENTION / CONTROL PROGRAM Bio – exclusion is a principle and discipline ( cont. ) l Clean hatching egg disinfection and handling. l Disinfection pit for every site / house. l People and any movable animal / element are the main carriers of the disease.

BIO – SECURITY MEASURES

Vehicles: BIO –SECURITY Farms l Minimize entry of vehicles to farm / sites. l Entry through single locked gate. l Fully disinfect vehicles, body, under -body, wheels, tires and driver cabin. l Veterinarian to specify disinfectants and their concentrations in footbaths and vehicles wheel dips. l The holding area of the farm trucks should be cleaned disinfected once/day.

BIO –SECURITY Farms Vehicles (cont. ): l Field services vehicles should not enter the sites and must be re-cleaned and disinfected in the services area after any trip. l Trucks used for culls, spent hens, and dead birds transfer, should be vigorously cleaned and fully disinfected before leaving the dispatch area.

BIO –SECURITY Farms Vehicles (cont. ): l Driver not to leave his cabin. If other wise, changing clothes, showering, using coverall supplied by the farm, after disinfecting the vehicle and the cabin carefully, is a must. l Disinfection pit should be large enough for vehicle wheels at each entrance gate. l Trucks should be disinfected at the main entrance and again at the farm gate.

People: BIO –SECURITY Farms l Closing , footwear and exposed body (eg. hair) can transmit the disease. l Periodical educational meetings for staff training. l Manager is responsible for overseeing entry and records of entry. l Keep a record of entry - visitors book

People (cont. ) : BIO –SECURITY Farms l Discourage visitors and minimize entry of people to farms. l Disinfect hands hair and shoes/overshoes before entering the 1 st barrier. l Poultry and poultry products not allowed to be entered. l Entry to sites through one single locked gate.

BIO –SECURITY Farms People ( cont. ) : l At Wadi 2 farms, second barrier station at which people should stop, redisinfect hands and shoes, fumigate bags, contents, change clothes, take a shower and use the farm clothes and shoes /slippers. l Keep the site record of entry - visitor book. l Clean and dirty areas of the whole farm must be defined and clearly identified.

BIO –SECURITY Farms People ( cont. ) : l To enter clean area of Noubaria farm, all visitors must disinfect hands, hair and shoes shower/change into farm clothing and use slippers / shoes. l To enter the clean area of the site all visitors and Wadi staff must disinfect hands, hair and shoes, shower again and change into shoes, underwear, overall and coat supplied by farm.

BIO –SECURITY Farms People (cont. ) : l In the breeder sites, slippers must be used outside sheds, to be changed to white plastic shoes upon entering the sheds. l Use a disnifection pit for every house. l Plastic shoes must be dipped in a disinfection footbath outside the shed, then dipped again in footbath inside the shed.

BIO –SECURITY Farms People (cont. ) : l Veterinarians to specify types and concentration of disinfectants for hands and footbaths. l Field service staff must audit on farm procedures. l All poultry staff and workers to report immediately any unusual levels of death or sickness to Wadi veterinarians / management.

BIO –SECURITY Farms Equipment and bedding material: l Clean /disinfect all equipment into farm using approved disinfectants and procedures. l Equipment includes : day old chick-boxes , pullet crates, egg trays, egg trolleys, shed dividers, vaccination and beak trimming equipment, scales, pullet boxes, maintenance equipment etc. . . l Each facility /station should be supplied with a high pressure disinfectant sprayer.

BIO –SECURITY Farms Equipment and bedding material (cont. ): l Each farm/site should have its own maintenance equipment. Special equipment to be cleaned and spray disinfected or fumigated or UV treated before entry. l Wood shavings ( nest and floor bedding material) from bird-proof storage areas , each batch should be fumigated with formaldehyde powder. l Move bedding material to the farm in tightly closed bags.

BIO –SECURITY Farms Equipment and bedding material (cont. ): l Nest bedding material to be treated with 5 gram powdered formalin / week / nest. l Each farm to be fully supplied with vaccination and post mortum utensils for its own use to be cleaned and disinfected after each use. l Thorough cleaning and disinfection of houses and equipment to be checked by vet. lab swabs results, followed by high level of quarantine and rest of two weeks period.

BIO – SECURITY Farms Spiking : l Males spiking from other farm/site is not allowed. l In - site males kept in a small house may be used for spiking when necessary

BIO – SECURITY Farms Culls and dead birds : l Dead birds on the farm(s) are to be hygienically buried on site in burial pits or to be transported inside clean closed bags to the vet. lab. or for disposal outside the farm / site. l Sexing errors or cull birds and table eggs are transported to the dedicated sales station, using dirty road only. l Keep all roads and spaces between sheds always clean. Disinfect them morning and afternoon daily using 3 % formalin.

BIO – SECURITY Farms Culls and dead birds ( cont. ) : l Dirty roads should be carefully cleaned after moving culls or spent hens during the dispatch period and to be sprayed 3 -4 times daily with 3 % formalin disinfectant. l Vigorous clean and spray - disinfect culls, spent hens , table eggs and dead birds dispatch area and pathways.

BIO –SECURITY Farms Rodents and insects : l Rodents and insects could carry AIV into sheds. l Remove any hiding materials around the farms sites. l Implement an effective rodent/ insects control program(feed, food, water and chemical poisons)in the farm sites/sheds and around staff housing. l Poultry houses must be surrounded with gravel.

BIO –SECURITY Farms Rodents and insects ( cont. ) : l Effectively close any shed holes to avoid rodent/ E insects entry and multiplication. l Avoid static water sources. l Avoid feed/egg wastage outside sheds. l Insure shed doors tightly closed to avoid entry of rodents / insects. l Use rodents / insects trapping. Mechanical rodents killing may assist.

BIO –SECURITY Farms Rodents and insects ( cont. ) : l Keep the daily dead birds of each shed in a plastic K bag, in a tightly closed container before transfer. l Cover all openings and feed silos and water storage tanks to prevent entry of rodents / insects. l Various, routinely changed , registered rodenticides / insecticides to be used according to producer instructions.

BIO –SECURITY Farms Domestic animals ( Dogs, cats, … ) : l Don’t permit any animal to enter site/ shed. l Insure fence height level and tightness to prevent wild animals entering the farms/sites. l Use wild animal hunting/trapping in the farm spaces between sites.

BIO –SECURITY Farms Domestic animals ( cont. ) : l Distribute food and water poisons for wild animal. l Disburse small water spots and avoid static water sources. l Avoid human food waste at the farm/site level. l Keep the dead birds in tightly closed plastic bags within a covered container until dispatch.

BIO –SECURITY Farms Grazing animals ( Sheep, goats, cows, … ) : l No grazing animals are allowed to be raised on the farm. l Don’t permit any animals to enter sites/sheds.

BIO –SECURITY Farms Wild domestic birds : l Healthy carriers /diseased or dead wild/domestic birds, waterfowl (particularly ducks and geese) poultry and feaces, are the main sources of infection/spread. l Periodically trim trees, destroy wild bird nests and burn them. l Don’t keep other birds or poultry on farms /sites.

BIO –SECURITY Farms Wild domestic birds ( cont. ) : l Impose one age group rearing per farm/site. l No vegetation between sheds, or near farm/site fences. l Minimize wild birds on farm by preventing and cleaning up feed spills. l Avoid small water spots and static water sources.

BIO –SECURITY Farms Wild domestic birds ( cont. ) : l Maintain shed wild bird netting and prevent roosting/ nesting on sheds. l Cover all openings to feed silos and water storage tanks to prevent entry of wild birds or birds feaces. l Keep the two doors of the shed closed. Upon entry close one first before the second one is opened.

BIO –SECURITY Farms Wild domestic birds ( cont. ) : l Destroy any sick/dead wild birds by burning before touching. l All equipment /bedding material that enters sheds to be stored in bird proof areas on farm/site. l Nest materials in storage, to be treated with formalin powder routinely once a week.

Water: BIO –SECURITY Farms l AIV can survive and be isolated from water. l Drinking, cooling water sources must be clean /cholrinated (3 -5 ppm) and in a totally enclosed system from the supply into the sheds. l Water used for showering must be treated with chlorine at (10 ppm)level. l Apply proper maintenance to the water pipes outside sheds and staff housing to avoid standing water spots.

BIO –SECURITY Farms erosols – dust infection : l Aerosol transmission to farm can occur if nearby poultry or other birds are infected with AI virus or from aerosolized infected feaces. l Regular disinfection of yards and walk ways and between houses. l Day old chicks are to be sourced from AI free certified GP flocks. l Dead birds on the farm are to be hygienically buried or to be transported inside clean bags and disinfected before leaving the site to the vet. lab or disposal pit.

BIO –SECURITY Farms erosols – dust infection ( cont. ) : l Main roads to be sprayed twice a day with 3% formalin solution. l Walk ways must be concrete for ease of cleaning and disinfection. l Sexing errors, spent or culled birds and table eggs should be transported to the sales station through the dirty roads only. l Dirty roads should be carefully cleaned during the disbatch period, and 3 -4 times (3% formalin) spray. l Approved end- of - batch cleaning/disinfection procedures are to be effective against AI virus , and recontamination of sheds is to be prevented.

BIO –SECURITY Hatchery ehicles : l Minimize entry of vehicles to the Hatchery. l Entry through single locked gate. l Fully disinfect vehicles body, under- body, wheels, tires and driver cabin. l Field services vehicles should not enter the hatchery and must be re -cleaned and disinfected in the services area after any trip.

BIO –SECURITY Hatchery ehicles ( cont. ) : l Veterinarian to specify disinfectants and concentration in footbaths, vehicles wheel dips, body and under- body spray. l Chick/hatching egg driver should use coveralls and plastic over shoes before leaving the hatchery and to immerse soles of shoes in disinfectant footbath. Use hand disinfection and put new cover shoes at the main entrance of the farm, to be changed through his trip back.

BIO –SECURITY Hatchery ehicles ( cont. ) : l Hatching egg trays and trolleys and truck body should be properly disinfected before returning to breeder farms. l Hatchery drivers should disinfect their vehicles, . cabin, hands and shoes before entering the hatchery premises again. l Driver not to leave his cabin. otherwise, changing clothes showering, using coverall supplied by the farm(after disinfecting the vehicle and the cabin carefully)is a must. l Disinfection pit at the entrance gate.

People : BIO –SECURITY Hatchery l Closing , footwear and exposed body (e. g. . hair) can transmit the disease. l Periodical educational meetings for staff training. l Manager is responsible for overseeing entry and records of entry. l Keep a record of entry - visitors book. l Discourage visitors and minimize entry of people to hatchery.

People (cont. ): BIO –SECURITY Hatchery l Entry to hatchery through one single locked gate. l Hands and footbath disinfection containers with suitable disinfection at each destination. l Use Ultraviolet boxes to disinfect items as glasses, watches , money and others at hatchery/showering entrance. l Clean and dirty areas of the whole hatchery are defined and clearly identified.

People (cont. ): BIO –SECURITY Hatchery l To enter clean area of the hatchery, all visitors and Wadi staff must shower/change into hatchery clothing and use hatchery /shoes. l Hands , hair and shoes disinfection after showering area. l Footbath at each destination. l Veterinarians to specify types and concentration of disinfectants for hand footbaths.

quipment : BIO –SECURITY Hatchery l Clean /disinfect all equipment into hatchery, using approved disinfectants and procedures. l Equipment includes : day old chick-boxes, egg trays, egg trolleys, vaccination/beak trimming equipment, scales, maintenance equipments etc. . . l Thorough cleaning and disinfection to be checked by vet. lab swabs results.

BIO –SECURITY Hatchery odents and insects : l Remove any hidden materials around the hatchery l Implement an effective rodent/ insects control program(feed, food, water and chemical poisons) in the hatchery and around staff housing. l Avoid static water sources. l Avoid egg waste outside dispatch area. l Use rodent / insect trapping. Mechanical rodents killing may assist.

BIO –SECURITY Hatchery omestic animals : l Don’t permit any animals to enter hatchery compound. l Insure fence height level and tightness to prevent wild animals entering the hatchery compound. l Distribute food and water poisons for wild animal. l Disburse water spots and avoid static water sources. l Avoid human food waste.

BIO –SECURITY Hatchery razing animals : Grazing l No grazing animals are allowed to be raised inside the hatchery compound. l Don’t permit any animals to enter the hatchery area.

BIO –SECURITY Hatchery Wild / domestic birds : l Healthy carriers /diseased or dead wild /domestic c birds, waterfowl (particularly ducks and geese) poultry and feaces, are the main sources of infection/spread. l Periodically trim trees, destroy wild bird nests and burn them. l Don’t keep other birds or poultry in the hatchery compound.

BIO –SECURITY Hatchery Wild / domestic birds (cont. ) : l No vegetation in the hatchery compound. l Maintain windows/openings bird netting and prevent roosting / nesting on hatchery roof. l Destroy any sick /dead wild birds by burning before touching.

Water: BIO –SECURITY Hatchery l AIV can survive and be isolated from water. l Drinking, cooling water sources must be clean /cholrinated (3 -5 ppm) and in a totally enclosed system from the supply into the hatchery. l Water used for showering must be treated with chlorine at 10 ppm level. l Apply proper maintenance to the water pipes outside the hatchery and staff housing to avoid standing water spots.

BIO –SECURITY Hatchery erosols – dust infection : l Aerosol transmission to hatchery can occur if nearby poultry or other birds are infected with AI virus or from aerosolised infected feaces. l Walk-ways should be concrete for ease of cleaning and disinfection. l Regular disinfection of yards and walk ways around the hatchery building. l Main roads to be sprayed twice a day with 3% formalin solution.

BIO –SECURITY Hatchery erosols – dust infection ( cont. ) : l Dead chicks and hatchery refuse are to be transported inside clean bags before leaving the hatchery to the disposal pit or to rendering. l Approved end- of - hatch cleaning / disinfection procedures are to be effective against AI virus and recontamination is to be prevented. l Thorough cleaning and disinfection to be checked by vet. lab. swabs results.

BIO –SECURITY Feed Plant ehicles : l Minimize entry of vehicles to the feed plant. l Entry through single locked gate. l Fully disinfect vehicles body, under- body, wheels, tires and driver cabin at feed plant gate. l Field services vehicles should not enter the feed plant and must be re-cleaned and disinfected in the services area after any trip.

BIO –SECURITY Feed Plant ehicles ( cont. ) : l Veterinarian to specify disinfectants and concentration in footbaths, vehicles wheel dips, body and under- body spray. l Feed plant driver should use coveralls and plastic over shoes before leaving the feed plant and to immerse soles of shoes in disinfectant footbath. Use hand disinfection and put new cover shoes at the main entrance of the farm, to be changed through his trip back.

BIO –SECURITY Feed Plant ehicles ( cont. ) : l Bulk feed preferable. Bagged feed in tightly closed bags. l Upon arrival to any farm driver not to leave his cabin. Otherwise, changing clothes showering, using coverall supplied by the farm, after disinfecting the vehicle and the cabin carefully, is a must. l Upon arrival to any farm trucks should be disinfected, at the main entrance and again at the farm gate.

BIO –SECURITY Feed Plant ehicles ( cont. ) : l Driver not to leave his cabin. Otherwise, changing clothes showering, using coverall supplied by the farm, after disinfecting the vehicle and the cabin carefully, is a must. l Disinfection pit at the entrance of the plant. l Trucks should be disinfected, at the main entrance and again at the farm gate.

People : BIO –SECURITY Feed plant l Closing, footwear and exposed body (e. g. . hair) can transmit the disease. l Periodically educational meetings for staff training. l Manager is responsible for overseeing entry and records of entry. l Keep a record of entry - visitors book

People (cont. ): BIO –SECURITY Feed plant l Discourage visitors and minimize entry of people to feed plant. l Entry to feed plant through one single locked gate. l Foot disinfectant pit at the entrance gate. l Veterinarians to specify types and concentration of disinfectants for hands and footbaths.

quipment : BIO –SECURITY Feed plant l Each facility /station should be supplied with a high pressure disinfectant sprayer. l New equipment should be subjected to disinfection at the feed plant gate.

BIO –SECURITY Feed plant odents and insects : l Remove any hidden materials around the feed plant. l Implement an effective rodent/ insects control program in and around the feed plant. l Distribute food, feed and water poisons. l Use rodent trapping. Mechanical killing may assist. l Cover all openings /feed silos and main stores to prevent rodent /insects entry and multiplication.

BIO –SECURITY Feed plant odents and insects ( cont. ) : l Avoid, clean and remove any raw material or feed waste. l Avoid feed /feed ingredients spillage outside silos. Keep stores tightly closed. l Regularly check insect infestation of grains in silos and apply appropriate insecticides whenever found.

BIO –SECURITY Feed plant omestic animals : l Don’t permit any animals to enter feed plant compound or stores. l Insure fence height level and tightness to prevent entry of wild animals. l Distribute food and water poisons for wild animals. l Disburse water spots and avoid static water sources. l Avoid human food and feed spillage within the feed plant compound.

BIO –SECURITY Feed plant razing animals : Grazing l No grazing animals are allowed to be raised inside the feed plant compound. l Don’t permit any animals to enter the feed plant area.

BIO –SECURITY Feed plant Wild / domestic birds : l Periodically trim trees, destroy wild bird nests and burn them. l Don’t keep any birds or poultry within the feed plant compound l No vegetation in the feed plant compound. l Minimize wild birds in feed plant by preventing /cleaning up feed spills l Avoid small water spots and static water.

BIO –SECURITY Feed plant Wild / domestic birds (cont. ) : l Healthy carries / diseased or dead wild / domestic birds, waterfowl ( particularly ducks and geese) poultry and feaces, are the main sources of infection / spread. l Maintain stores/silo bird netting and prevent roosting / nesting on feed plant stores or silo roofs. l Cover all openings to feed silos and water storage tanks to prevent entry of birds or birds feaces.

BIO –SECURITY Feed plant Wild / domestic birds (cont. ) : l Destroy any sick / dead wild birds by burning before touching. l Apply daily morning and afternoon time, 3%formalin spay the plant roads and walkways.

Water: BIO –SECURITY Feed plant l AIV can survive and be isolated from water. l Drinking, cooling water sources must be clean /cholrinated (3 -5 ppm) and in a totally enclosed system from the supply into the feed plant. l Apply proper maintenance to the water pipes outside the feed plant and staff housing to avoid standing water spots.

BIO –SECURITY Feed plant erosols – dust infection : l Aerosol transmission to feed plant can occur if nearby poultry or other birds are infected with AI virus or from aerosolised infected feaces. l Walk-ways should be concrete for ease of cleaning and disinfection.