Synthetic Biology Review A Synthetic Oscillatory Network of
Synthetic Biology Review A Synthetic Oscillatory Network of Transcriptional Regulators – ‘The Repressilator’ Elowitz, M. B. & Leibler, S. Nature, vol. 403, 20 th January 2000 _______________________ Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 1 of 6
The Basics _______________________ Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 2 of 6
The Good Results. . . _______________________ Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 3 of 6
. . . and the Other Results ◄ Limitations (c): » Accumulation of GFP as a result of limited elimination » Green – oscillation with a reduced period » Blue – several periods skipped entirely ◄ Controls (f): » Cells containing only the reporter plasmid Stochastic effects… _______________________ Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 4 of 6
Conclusions & Extensions ◄ i. GEM Solution: A Lux R Plux » Novel approach based on Lotka-Volterra model of predator-prey interaction Lux I A Cell A » Prey cell is repressed by product of predator cell… Cell B Lux. R A Plux » Constitutively repressed ‘predator’ cell is anti-repressed by product of ‘prey’ cell A Lux. R Aii. A Lux R aii. A » Thus a stable oscillation cycle is built up _______________________ Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 5 of 6
The Network Model Deterministic Continuous / Stochastic Discrete Continuous model: _______________________ Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 6 of 6
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