Synaptogenesis Illustrations from Development of the Nervous System
- Slides: 33
התפתחות סינפסות Synaptogenesis Illustrations from: Development of the Nervous System/ Sanes, Rea, Harris 1 Golan H. 2010
Morphological evidences – detectable just in relative advance stage of synapse formation - EM. 3 Golan H. 2010
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Physiological evidences – Synapse function 6 Golan H. 2010
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Synapse functional maturation: Presence of neurotransmitter synthesis and release machinery Before synaptogenesis. Neurotransmitter release – occur before synapse formation – Mechanism mostly vesicular. 10 Golan H. 2010
Ca dependency Growth cone And Synapse (at all ages) 11 Golan H. 2010
Adhesion 12 Golan H. 2010
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From growth cone to presynaptic terminal microtubules frizzled b-Nerexin Vesicles Neuroligin-1 15 Golan H. 2010
The elements of the PNS synapses 16 Golan H. 2010
Innervation of muscle cell by the motor neuron induces: Increase in the number of receptors Increase in the amount of receptors clustering *Specificity 17 Golan H. 2010
Neural Agrin or muscle Agrin Induces receptor clustering? 18 Golan H. 2010
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Agrin binds to a receptor complex and Ma. SK is required for clustering 20 Golan H. 2010
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The elements of the CNS synapses 22 Golan H. 2010
Eph. B signaling influences Glutamate receptors aggregation In the CNS 23 Golan H. 2010
Eph -Rs 24 Golan H. 2010
White – glut application Yellow – size of synaptic currents Green – synaptic contacts 25 Golan H. 2010
Neural activity Regulation of receptor expression Regulation of post and presynaptic transcription Synaptic strength 26 Golan H. 2010
Activity dependent …. . 27 Golan H. 2010
The duration of synaptic potentials decreases during development 28 Golan H. 2010
Receptors subunits – regulation with age In all receptor types Depends on: Age Brain region Activity Chemiacl interactions (trophic factors) 29 Golan H. 2010
Glutamatergic synapse maturation 30 Golan H. 2010
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Murai et al, 2003 Eph A 3 on astrocyte – red Synaptophysin – blue YFP- dendritic spine - green A role of astrocyte in The regulation of spine shape 33 Golan H. 2010