Sympathetic denervation in the mice My life in
- Slides: 23
Sympathetic denervation in the mice ~ My life in San Diego 生科 04 楊慧儒 891644 2002. 12. 06
劉新光 教授 John H. K. Liu, Ph. D. n n the regulation of intraocular pressure An elevated intraocular pressure ─ primary open-angle glaucoma the circadian (24 -hour) rhythms of intraocular pressure and related ocular functions. Human study─ eye drop for glaucoma
Summer research n n n Superior cervical ganglionectomy Way to measure the pupil size of the mice Test of eye drop on postsurgical mice
Superior Cervical Ganglion (SCG)
Superior Cervical Ganglion (SCG) n n Sympathetic nerve─change in pupillary reflex Removal or sectioning of this part can reduce the circadian IOP elevation The outcome of removal of SCG or cutting the cervical sympathetic trunk will mimic human Horner’s syndrome─drug test PAST~ n n Palpebral ptosis and become irreversible from 20 th hr Palpebral retraction
Pharmacology of the sympathetic nervous system hydroxyamphetamine
Drug to Test Horner’s Syndrome Hydroxyamphet Dilation→ Postganglio aine(HA) no dilation nic & Tyramine(TA) Cocaine(C) Dilation→ Pre-, Epinephrine(E) No dilation postganglio nic No dilation→ Postganglio Dilation nic
Ganglionectomy Toe & tail Mouse(C 57 BL/6) Male, 30 g, 4 mice Anesthesia Surgery (Ketamine+Xylazine ) Fix it on the bench and preparation of instruments
Ganglionectomy n n n Shave Incision Find SCG Cut SCG (right side) Suture back the fats & the skin
Ganglionectomy
Measurement of pupil size n Anesthetized n n n Ketamine pentobarbital Conscious n n Tube Cone n Ruler
Eye Drop Test n n Solution (saline) 1 micro-liter, twice, on each eye Record every 5 -10 mins Experimental time: 70 mins n n n M: mouse number M 0: normal mouse (day): the day after surgery
Result. Hydroxyamphetamine & Tyramine HA (1%) Conscious Ketamine Pentobarbital M 1(9), M 2(8), M 0 M 1(7), M 2(6) M 1(10) HA (0. 1%) M 2(7) TA (5%) M 1(15), M 2(14), M 3(7), M 0 TA (1%) M 1(16), M 2(15), M 3(8) TA (0. 1%) M 1(17), M 2(16), M 3(9)
Result- Cocaine Conscious Cocaine M 1(18), M 2(17), M 4(1, 3), M 0 Pentobarbital M 2(9)
Result- Epinephrine Conscious Epinephrine 0. 1% M 1(10) M 2(13) M 3(6, 10, 15) M 4(8) M 0
Discussion n Compare to rabbits: n n Cocaine─ 5~30 mins Hydroxyamphetamine─ 30~45 mins Cocaine, hydroxyamphetamine, and tyramine work on rabbits but not mice. Only using epinephrine at certain day after surgery(10 th-15 th days) can have significant result to confirm the success of surgery.
Furthermore n n Increase the number of experiment Find a critical way to measure the pupil size of mice Electrode stimulation The mechanism of drug effect
Life in San Diego
Life in San Diego Balboa park
Life in San Diego Mission bay & Balboa park
University of California, San Diego
Campus
- Barostim
- Mark wholey md
- Verbal communication
- Visceral autonomic nervous system
- Parietal mesoderm
- Terminal ganglia
- Beta-blockers for overactive sympathetic nervous system
- Sympathetic parasympathetic
- Difference between sympathetic and parasympathetic
- Diff between sympathetic and parasympathetic
- Sympathetic drugs
- Ans
- Kerja saraf simpatik dan parasimpatik
- Sympathetic and parasympathetic nervous system difference
- Sympathetic innervation of the heart
- Patellar reflex
- Sympathetic v parasympathetic
- Author's tone and bias
- Adrenergic fibers sympathetic or parasympathetic
- Cervical sympathetic
- Cervical sympathetic
- Prevertebral ganglia
- Sympathetic dialogue
- Adrenal sympathetic pathway