Supramolecular Nano Stamping 2 Molecular Electronics Nano Bio
Supramolecular Nano. Stamping
2 Molecular Electronics
Nano Bio Robots 3
Nanobio Sensors 4
Top Down 5
Top Down Nano imprinting 6
7 Bottom up Dip Pen Nano-lithography
8 Bottom up • Self assembly • Selectivity
9 Supramolecular Interaction § § § Hydrogen Bonding Metal Coordination Hydrophobic Forces Van der Waals Forces pi-pi Interactions Electrostatic Effects
10 The Supramolecular Nano. Materials Group Francesco Stellacci
11 Master Supramolecular Interaction Stamping
DNA Micro Array 12 ü Identifying disease ü Finding out proper treatment for disease ü Measuring the effects of a specific drug on a particular set of genes
13 In-Situ Fabrication Of DNA Microarray
14 Su. NS Process Details 3 Step Process 1. 2. 3. Hybridization Stamping Dehybridization
3 Step Printing Cycle Step 1 : Hybridization Cleaning in HS-ssc. DNA Solution Immersion Rinse 1 HS-c. DNA : low concentration 5 umol solution buffer solution(0. 1 M Na. Cl/TE) Rinse De. Ionized Water Buffer 2: : 0. 5 M Na. Cl/TE buffer, with p. H 7 Master 15
3 Step Printing Cycle Step 2 : Stamping An Extra Step : Drying Contact Approacehs Additional drying step introduced to provide Higher resolution. a. Capillary Approach b. Mechanical Approach 16
3 Step Printing Cycle Step 3: De. Hybridization Master and Printed copy separated by i. Increasing Temperature : 75 -80 o. C ii. Mechanical Force : Fdehyb< F surface-bond 17
18 The microarray obtained is the mirror image of the template TEMPLATE Molecular Stamping MS MICROARRAY
19 The Complete Printing Cycle
20 Credibility of This Process • Thinnest printed pattern line obtained using 18 mer and 50 mer are 22 nm and 38 nm respectively. • Replication of DPN fabricated microarray DPN-Dip Pen Nano. Lithography
21 Problems of Gold Substrate • Opaque • Rigid • Requirement of complex facilities • Expensive
PMMA : Poly. Methyl. Meth. Acrylate A popular acrylic polymer often used as a replacement for glass Advantages : a. Optically transparent b. Inexpensive (1/70, 000 of gold cost) c. Conformal contact 22
23 Advancement Liquid Su. NS (Li. Su. NS) : • New derivative of Su. NS that can handle any roughness of substrates • Use liquid prepolymer solution as a secondary substrate to be printed. Prepolymer c. DNA Hybridization DNA Gold Master Cured Prepolymer Dehybridization Gold Master Cured Prepolymer Gold Master
24 Credibility Able to copy from masters fabricated by : • Different lithography • Inkjet printer copies
25 Advantages of Su. NS • • • Serial vs. parallel printing technique Best resolution Larger coverage Faster and Simpler Process Higher Information transfer
26 Future Prospects • Applicable to any pairs of molecules that can recognize and attach to each other • Printing can be done with other things like proteins, antibodies and viruses • Extension in semiconductor field to produce devices such as metallic wires, single-electron transistors, optical biosensors, micro and nano fluidics channels etc. • Large memory storage
27 Top Job of bio engineers (A hot field) BIOMEDICAL ENGINEER Job Growth: 72 percent, or 12, 000 new jobs by 2018 Salary: $82, 550 mean; $103, 000 for scientific and technical consultants
28 Thank You!
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