Supplementary Table 2 Conjugation efficiencies of Ll Ltr

Supplementary Table 2 Conjugation efficiencies of Ll. Ltr. B variants mutated at DI Intron Variants Conjugation Efficiencies Standard Errors Base Pairs Ltr. B ORF 1. 43 E-02 1. 03 E-02 16 Ltr. B ORF-0 1. 26 E-08 1. 21 E-09 0 Ltr. B ORF-5 7. 43 E-07 1. 29 E-07 5 Ltr. B ORF-13 5. 58 E-02 2. 40 E-02 13 S 8 - ORF 2. 05 E-04 1. 03 E-04 16 S 8. 0 - ORF 1. 09 E-08 8. 40 E-09 0 S 8. 1 - ORF (tcw) 3. 14 E-09 1. 35 E-09 6 S 8. 2 - ORF (tcw) 3. 06 E-09 1. 30 E-09 11 S 8. 3 - ORF (tcw) 5. 10 E-09 3. 20 E-09 13 S 8. 4 - ORF (fcw) 2. 28 E-09 1. 90 E-10 5 S 8. 5 - ORF (fcw) 3. 21 E-06 1. 50 E-06 10 S 8. 6 - ORF (fcw) 4. 41 E-06 1. 80 E-06 13 Conjugation efficiencies of Ll. Ltr. B variants mutated at DIII Intron Variants Conjugation Efficiencies Standard Errors Base Pairs Ltr. B ORF 1. 43 E-02 1. 03 E-02 17 Ltr. B ORF-0 1. 55 E-08 8. 24 E-09 0 Ltr. B ORF-5 9. 36 E-07 3. 04 E-07 5 Ltr. B ORF-14 2. 33 E-02 6. 36 E-03 14 S 5 - ORF 2. 12 E-04 1. 65 E-04 17 S 5. 0 - ORF 7. 51 E-09 1. 16 E-09 0 S 5. 1 - ORF (tcw) 5. 39 E-09 9. 70 E-10 6 S 5. 2 - ORF (tcw) 6. 31 E-08 2. 47 E-08 11 S 5. 3 - ORF (tcw) 8. 73 E-08 8. 90 E-09 17 S 5. 4 - ORF (fcw) 1. 78 E-07 3. 04 E-08 5 S 5. 5 - ORF (fcw) 8. 72 E-08 3. 70 E-08 11 S 5. 6 - ORF (fcw) 9. 70 E-08 1. 00 E-08 14

Conjugation efficiencies of Ll. Ltr. B variants mutated at DIVb Intron Variants Conjugation Efficiencies Standard Errors Base Pairs Ltr. B ORF 1. 43 E-02 1. 03 E-02 16 Ltr. B ORF-0 1. 91 E-05 4. 94 E-06 0 Ltr. B ORF-8 3. 80 E-02 5. 49 E-03 8 Ltr. B ORF-12 7. 70 E-02 1. 63 E-02 12 S 3 - ORF 2. 05 E-03 4. 20 E-04 15 S 3. 0 - ORF 8. 68 E-08 2. 97 E-08 0 S 3. 1 - ORF (fcw) 2. 63 E-08 1. 29 E-08 4 S 3. 2 - ORF (fcw) 3. 16 E-07 2. 38 E-07 8 S 3. 3 - ORF (fcw) 5. 43 E-06 1. 07 E-06 12 S 3. 4 - ORF (fcw) 3. 96 E-05 2. 72 E-05 15 Conjugation efficiencies of Ll. Ltr. B variants mutated at DIVa Intron Variants Conjugation Efficiencies Standard Errors Base Pairs Ltr. B ORF 1. 43 E-02 1. 03 E-02 16 Ltr. B ORF-0 9. 69 E-06 7. 81 E-06 0 Ltr. B ORF-8 1. 60 E-04 4. 54 E-05 8 Ltr. B ORF-12 1. 69 E-03 6. 35 E-04 12 S 2 - ORF 3. 22 E-04 1. 07 E-04 16 S 2. 0 - ORF 1. 19 E-07 1. 01 E-07 0 S 2. 1 - ORF (tcw) 1. 48 E-07 1. 28 E-07 4 S 2. 2 - ORF (tcw) 1. 01 E-06 2. 20 E-07 8 S 2. 3 - ORF (tcw) 4. 05 E-05 8. 30 E-06 12 S 2. 4 - ORF (tcw) 2. 44 E-04 2. 60 E-05 16