Supplementary Effect of Solvent Concentration on Total Flavonoid

Supplementary Effect of Solvent Concentration on Total Flavonoid and Total Phenolic content of Averrhoa bilimbi Leaf Extract

Extraction with percolation

Averrhoa bilimbi leaf extract

Table 1. The result of yield Averrhoa bilimbi extract No Solvent (%) Weight of Extract dry leaf (gram) Randemen (%) 1 Ethanol 50 60 14, 4 24 2 Ethanol 70 60 25, 2 42 3 Ethanol 96 60 13, 1 21, 83

Table 2. The result of phytochemical screening Averrhoa bilimbi extract. Compound Extract Ethanol of Averrhoa bilimbi leaf 50% 70% 96% Flavonoids + + + Phenolic (tannin) + + + Alkaloids + + Observations The occurance of a red or orange colouration A blue coloration resulting from the addition of ferric chloride reagent + orange-brown spots on a yellow background with Dragendroff reagent Saponins + + + A peristent foam is formed above the liquid surface Steroids + + + A reddish brown colour at the interface

Table 3. The result of Thin Layer Chromatoghrapy Averrhoa bilimbi extract Comound Solvent (Ethanol) 50% Mobile Phase 70% Alkaloids Observations (+) orange-brown spots on a yellow background with Dragendroff reagent [2] Me. OH-NH 40 H (200: 3) 96% (-) 50% Flavonoids 70% n-Butanol-Acetid acid-water (4: 1: 5) 96% 50% Saponins 70% chloroform-methanol-water (13: 7 : 2) 96% 50% Phenolic Retradition Factor (-) (+) (Rf = 0, 84) 70% Acetid acid-chloroform (1: 9) 96% 50% 70% Steroids hexane-ether (97: 3) 96% (+) (Rf 1 =0, 16) (Rf 2=0, 88) (+) (Rf 1 = 0, 15) (Rf 2 = 0, 88) (+) (Rf =0, 17) (+) Bright yellow [2] (+) (Rf 1=0, 5) (Rf 2=0, 14) (+) (Rf=0, 12) (+) (Rf=0, 11) (+) (Rf 1 = 0, 16) (Rf 2=0, 95) (+) (Rf = 0, 11) (Rf=0, 89) (+) (Rf 1 = 0, 11) (Rf 2=0, 93) (-) (+) (Rf 1 =0, 72) (Rf 2=0, 92) (+) (Rf =0, 57) (Rf 2=0, 82) (Rf 3=0, 88) (Rf 4=0, 89) (Rf 5=0, 93) (Rf 6=0, 96) (+) pink to purple spots [2] (+) Blue spots [2] (+) orange colour, with a green fluorescence in UV. [2]

Table 4. TFC and TPC of Averrhoa bilimbi extract Ethanol extract TFC (mg RTE/g) TPC (mg GAE/g) 50% 62, 7358± 1, 1599 103, 7943 ± 3, 1854 70% 64, 8135 ± 1, 8533 119, 4707 ± 1, 7617 96% 59, 1035 ± 0, 3302 110, 1000 ± 1, 7324

Figure 1. Reactions between Rutin with Al. Cl 3

Figure 2. The curve of the relationship between absorbance vs concentration of TFC

Figure 3. Reaction of phenol compounds with Folin-Ciocalteu reagents

Figure 4. The curve of the relationship between absorbance vs concentration of TPC

Proposed Reviewers Name Affiliation E-mail Prof. Dr. Abdul Rohman, M. Si. , Apt Universitas Gadjah Mada abdulkimfar@gmail. com