Supplemental Diglta Content 1 Figure 1 a Effector

Supplemental Diglta Content 1 _ Figure 1 a/ Effector T cells (Th-) Regulatory T cells Treg RORgt FOXP 3 ns Th 17 * Tbet *** ** TGFb *** IL 17 A ** IL 10 *** *** ns IL 21 ns *** *** IL 4 ns ns *** TIM 3 ns *** ns ns *** IFNg *** Th 2 GATA 3 ns ns *** ns Th 1 * ***

Supplemental Digital Content 1 – Figure 1 b/ ns MEDIUM MPA (3µg/m. L) TACROLIMUS EVEROLIMUS (5µg/L)

Supplemental Digital Content 1 - Figure 1: Differential effects of MPA, tacrolimus and everolimus upon human CD 4+ T cells in vitro polarization and IL 17 production. a/ Effects of MPA, tacrolimus and everolimus upon the transcription of CD 4+ cell subsets-related genes after two days of T cell activation. CD 4+ T cells were activated following the same protocol, alone or in the presence of MPA and tacrolimus at a final concentration of 3µg/m. L and 5µg/L, respectively. A comparative RT-PCR of the relative expression of CD 4+ T cell subsets-related genes was then realized. *, P<0. 05; **, P <0. 01; ***, P <0. 005 (Comparison of means, Wilcoxon signed-rank test, . Prism 5; Graph Pad Softwares, San Diego, Calif). Bars represent medians. b/ Effects of MPA, tacrolimus and everolimus upon IL 17 secretion. CD 4+ T cells from 18 healthy volunteers were activated for 48 hours alone or in the presence of MPA and tacrolimus at a final concentration of 3µg/m. L and 5µg/L. Frequency of IL 17 -producing CD 4+ T cells among 50 000 activated CD 4+ T cells was measured by ELISpot. The figure presents the comparison of the frequency of IL 17 A. *, P<0. 05; **, P <0. 01; ***, P <0. 005 (Comparison of means, Wilcoxon signed-rank test, . Prism 5; Graph Pad Softwares, San Diego, Calif). Bars represent medians.