Sup Figure 1 Aki 1 EGFR pCREB tCREB
Sup. Figure 1 Aki 1 EGFR p-CREB t-CREB βactin 800 600 400 700 % cell viability 1000 Scramble Medium si. NC si. Aki 1#1 si. Aki 1#2 si. EGFR#1 si. EGFR#2 211 H 600 500 400 300 100 0 0 Day 2 Day 3 Day 4 si. EGFR#2 si. EGFR#1 si. Aki 1#2 si. Aki 1#1 Medium Scramble si. NC si. Aki 1#1 si. Aki 1#2 si. EGFR#1 si. EGFR#2 H 2052 200 Day 1 Scramble Medium si. EGFR#2 si. EGFR#1 si. Aki 1#2 si. Aki 1#1 H 2052 Aki 1 EGFR 1200 % cell viability Scramble Medium 211 H Day 1 Day 2 Day 3 Day 4
Aki 1 t-CREB βactin si. CREB 1#2 si. CREB 1#1 si. Aki 1 H 290 Scramble si. CREB 1#2 si. CREB 1#1 H 2052 si. Aki 1 Scramble si. CREB 1#2 si. CREB 1#1 211 H si. Aki 1 Scramble si. CREB 1#2 si. CREB 1#1 si. Aki 1 Scramble Sup. Figure 2 Y-meso 14
Sup. Figure 3 Met-5 A 120 % cell viability 100 80 60 40 20 Scramble si. Aki 1 si. CREB 1#2 0 Aki 1 t-CREB β-actin
Sup. Figure 4 Aki 1 P PKA EGFR Akt P CREB 1 Cyclin. A Cell signal
Sup. Figure 5 p-CREB 0 (negative) 1 (low) 2 (intermediate) 3 (high) Aki 1
Sup. Figure 6 A p-CREB score 0(negative) 1(low) 2(intermediate) 3(high) Positive/total (%) Epithelioid 1 2 9 9 20/21 (95) Sarcomatoid 4 1/5 (20) Desmoplastic 1/1 (100) Biphasic 1 5 6/6 (100) Other 2 2/2 (100) Total 5 2 12 16 30/35 (86) Aki 1 score 0(negative) 1(low) 2(intermediate) 3(high) Positive/total (%) Epithelioid 16 12 7 37/37 (100) Sarcomatoid 2 3 3 2 8/10 (80) Desmoplastic 1 4 2 6/7 (86) Biphasic 4 7 1 12/12 (100) Other 1 2/2 (100) Total 3 28 22 15 65/68 (95) Epitheloid type 3 Aki 1 score (0 -3) 3 2 1 N=5 r=0. 365 (Spearman rank) p=0. 6 2 1 0 0 2 3 0 1 p-CREB score (0 -3) Biphasic type Sarcomatoid type N=21 r=0. 561 (Spearman rank) p=0. 009 3 Aki 1 score (0 -3) B 2 3 0 1 p-CREB score (0 -3) N=6 r=0. 424 (Spearman rank) P>0. 999 2 1 0 2 3 0 1 p-CREB score (0 -3)
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