Summer Writing Workshop Robert Williams 1 Original Passage

Summer Writing Workshop Robert Williams 1

Original Passage While overexpression of a single transcription factor alone is insufficient to induce reprogramming of germ cells, ectopic expression of the neuronal selector zinc finger transcription factor CHE-1, in combinations with RNAi knockdown of LIN-53, the worm homolog of the histone-binding protein Rb. Ap 46/48, which participates in chromatin remodeling, results in directed conversion of germ cells into ASE-like gustatory neurons Spickard EA, Joshi PM, Rothman JH. The multipotency-to-commitment transition in Caenorhabditis elegansimplications for reprogramming from cells to organs. FEBS Lett. 2018; 592(6): 838 -851. doi: 10. 1002/18733468. 12977 58 words!!! �� 2

Sentence Logic Diagram While overexpression of a single transcription factor alone is insufficient to induce reprogramming of germ cells, ectopic expression of the neuronal selector zinc finger transcription factor CHE-1, in combinations with RNAi knockdown of LIN-53, the worm homolog of the histone-binding protein Rb. Ap 46/48, which participates in chromatin remodeling, results in directed conversion of germ cells into ASE-like gustatory neurons. 3

Things to consider for long sentences • From Writing Science by Joshua Schimel • Where is the stress, is it in the right place? • what is the topic, is it introduced early enough? • what is the story arc, is it in the right order? • Things I usually ask myself • Does all this information need to be in one sentence? • Is there a way I can break up this idea into more manageable pieces? 4

Revised Passage Overexpression of a single transcription factor alone is insufficient to induce reprogramming of germ cells. Directed conversion of germ cells requires combinatorial perturbation. For example, the combination of ectopic expression of the neuronal fate transcription factor CHE-1, and RNAi knockdown of chromatin remodeling factor homolog LIN-53 results in germ cell conversion to ASE-like gustatory neurons. 5