Summer School in NCSR Demokritos Summer 2005 Methods

Summer School in NCSR Demokritos Summer 2005 Methods in micro – nano technology and nanobiotechnology Organizer: National Center for Scientific Research “Demokritos” Participating Institutes: Microelectronics, Radioisotopes and Radiodiagnostic Products, Physical Chemistry Information: www. imel. demokritos. gr, Contact: Evangelos Gogolides, evgog@imel. demokritos. gr

Rational: Modern Research takes advantage of Micro and Nanotechnology developments. Merging areas of research (Nanobiotechnology) demand interdisciplinary skills. Necessary for researchers from Life Sciences, Chemistry, and Engineering to acquire skills in Micro and Nanotechnologies. Target: Establish common language between the various disciplines-promote interdisciplinary research Content: Five (5) day intensive summer school Offers: classroom and laboratory experience on: micro and nano-technology processes / materials / applications Targeted in: Nanobiotechnology

Who should attend: Group leaders involved in molecular biology or biotechnology, Post Doctoral Fellows Graduate students with Engineering, Science or Life Science background, All those who wish to apply micro-technology in their research. Maximum number of registrants 20 persons. Fees: 1000 Euro (accommodation NOT included) Dates: 6 -10 June 2005. Synergies with other schools in Greece and Nano 2 life: Back to back with Practical Course on Advanced techniques in molecular biology organized by FORTH/ICEHT, Date: 13 -18 June 2005 http: //mb_school. iceht. forth. gr A small reduction in the fee for those taking both seminars is being considered.

Syllabus 1: Lecture 2: Introduction to micro and nano-technology and nanobiotechnology Patterning technologies (lithography and plasma etching) for Si, glass, and plastic substrates in the fabrication of analytical/bioanalytical microdevices Laboratory 1: Fabrication of microfluidic devices on plastic substrates by novel lithographic techniques.

Syllabus 2: Lecture 3: Principles of Integrated Biosensing Devices Laboratory 3: Operation of a lab-on-a-chip optical device using model assays and real time measurements Monolithic silicon optocouplers PMMA Capillaries

Syllabus 3: Lecture 4: and biomolecules of Patterning substances ays ein Laboratory 4 a: of Fabrication A: Laboratory 4 b: Part biocompatible photoresist-based lithography. Part B. Fluorescence detection of protein arrays. Twelve rows of different protein spots fabricated in 12 succesive lithographic steps Fluorescence picture of the rabbit γ-globulins and biotinylated-BSA spot arrays after a 2 h immunoreaction with a mixture of AF 546 labeled streptavidin (red spots) and AF 488 labeled anti-rabbit Ig. G antibody (green spots). The spot size is approximately 4 μm.

Syllabus 4:

Syllabus 5: Lecture 7: Drug Delivery, Liposomes, Dendrimers, Cyclodextrins Laboratory 7: Dynamic light scattering and Z-potential measurements, Video enhanced optical microscopy of Liposomes and Atomic Force Microscopy Formation of DNA nanoparticles of ~40 nm diameter

Syllabus 6: Laboratory 7: Dynamic light scattering optical microscopy 5μm 5μm PC-CHOL-ODPG / DHP Liposomes PEG 0% PEG 5% PEG 15% 5μm 5μm Liposome-liposome interactions Correlation of Optical Microscopy and Dynamic Light Scattering results

Syllabus 7: Laboratory 8: Drug inclusion monitoring in situ by NMR spectroscopy X-ray diffraction characterisation of drug inclusion in Cyclodextrins and 3 -D visualisation NH 2 (OH)n 6