Structures of the Purine and Pyrimidine Bases Example
Structures of the Purine and Pyrimidine Bases
Example of the Structure of a Nucleotide Base Nucleoside Nucleotide
Chemical Structure of a Nucleic Acid Voet Fig. 3 -6
Double Helical Structure of DNA Note that the strands run antiparallel Note that the helix is 20 Å wide. There about 10 base pairs per turn of the helix. One turn of the helix is 34 Å The base pairs are 3. 4 Å apart Voet Fig. 3 -9 Major groove
Base Pairing Voet Fig. 23 -1
Many proteins associate With DNA through the Major and Minor grooves Major Groove http: //www. clunet. edu/Bio. Dev/omm/gallery. htm
Space-filling Model of B-DNA Backbones of the strands are bright green and bright red The bases on the corresponding backbones are lighter green and pink
Helical Structures A-DNA B-DNA Z-DNA Voet Fig. 23 -2
Supercoiling of DNA Supercoiled DNA Relaxed DNA
Denaturation of DNA Voet Fig. 23 -15
Restriction Endonucleases Voet Fig. 3 -18
Restriction Digest Cutting DNA with Restriction Endonucleases followed by Analysis by Gel Electrophoresis A C B
Visualizing DNA Fragments in Gels Voet Fig. 3 -20
Southern Analysis Voet Fig. 23 -28
Chain Termination Method of DNA Sequencing Voet Fig. 3 -23
DNA Sequencing Instruments Fluorescent dye-conjugated derivatives of the Dideoxynucleotides can be used in DNA Sequencing Instruments
Automated Sequencing Example of data obtained from a DNA sequencing reaction using fluorescent (dye-conjugated) derivatives of dideoxynucleotides Voet Fig. 3 -25
Polymerase Chain Reaction Amplification of DNA Garrett and Grisham Fig. 13 -21 See Voet Fig. 3 -32
Plasmids can Serve as Cloning Vehicles (Vectors) Voet Fig. 3 -27
Construction of a Recombinant DNA Voet Fig. 3 -29
Selection of Transformants Vector DNAs must be capable of replicating in the host cells and must carry a selectable marker
Selection of Transformants of Interest Voet Fig. 3 -31
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